Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima

master's thesis

• Title (english): Optimization of growth medium for maintaining bacteria of genus Bacteroides in vitro
• Author: Sanja Galić
• Mentor: Jurica Žučko (mentor)
• Committee member: Janko Diminić (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2020-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Zbog važnosti utjecaja na zdravlje čovjeka crijevna mikrobiota postala je često područje istraživanja. Budući da je provođenje istraživanja na ljudima iznimno kompleksno, sve se više razvijaju in vitro modeli za simulaciju uvjeta u ljudskome probavnom sustavu. Svrha ovog rada bila je u već razvijenom in vitro sustavu testirati utjecaj SIEM (eng. Simulated Illeal Efflux Medium) hranjive podloge na održavanje sastava inokulirane crijevne mikrobiote. Uzgoj crijevne mikrobiote u in vitro sustavu korištenjem SIEM hranjive podloge rezultirao je drastičnim padom udjela pripadnika koljena Bacteroidetes, te je predložena optimizacija hranjive podloge na osnovi podloga koje se koriste za uzgoj bakterija koje pripadaju rodu Bacteroides – najzastupljenijem rodu koljena Bacteroidetes u crijevnoj mikrobioti čovjeka.

Abstract (english)

Due to its importance for human health, the intestinal microbiota has become popular topic of research. Since conducting research on humans is demanding and complex new in vitro models are increasingly being developed to simulate conditions in the human gastrointestinal tract. The aim of this thesis was to test the influence of SIEM (Simulated Illeal Efflux Medium) growth medium on the viability of the inoculated microbiota composition in previously developed in vitro system simulating lower gastrointestinal tract. Cultivation of intestinal microbiota in vitro using the SIEM medium had resulted in drastic decline of members of the Bacteroidetes phylum. Optimization of SIEM growth media was proposed based on growth media used for cultivating bacteria of genus Bacteroides - most abundant genus of Bacteroidetes phylum in human gut microbiota.

• Keywords: crijevna mikrobiota; SIEM; Bacteroidetes; optimizacija; Bacteroides
• Keywords (english): gut microbiota; SIEM; Bacteroidetes; optimization; Bacteroides
• Language: croatian
• URN:NBN: urn:nbn:hr:159:088128
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-15 10:09:28