Utjecaj bioaktivnih molekula iz ekstrakta cvijeta Prunus spinosa L. na hiperglikemiju u miša

master's thesis

• Title (english): The influence of bioactive molecules from Prunus spinosa L. flower extract on hyperglycaemia in mice
• Author: Tajana Frančić
• Mentor: Irena Landeka Jurčević (mentor)
• Committee member: Verica Dragović-Uzelac (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2020-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Brojna istraživanja pokazuju da dijeta bogata polifenolnim spojevima pomaže u regulaciji hiperglikemije. Ekstrakt cvijeta Prunus spinosa L. promatran je zbog bogatog polifenolnog sastava, te utjecaja na razinu inzulina, glukoze u krvi (GUK), α-amilaze i nastalih produkata oksidacijskih proteina (AOPP) kod C57BL/6 miša. Kod miševa je izazvana hiperglikemija pomoću aloksana (150 mg kgˉ¹ tjelesne mase) te su tretirani sa ekstraktom cvijeta Prunus spinosa L. u dozi od 25 mg kgˉ¹ tjelesne mase koji je bogat bioaktivnim molekulama. Eksperiment je trajao 10 dana na miševima podijeljenima u četiri grupe. 1. grupa je kontrolna (K), 2. grupa je tretirana ekstraktom cvijeta Prunus spinosa L. (ECT), 3. grupa je aloksan grupa (AL), 4. grupa je aloksan grupa tretirana ekstraktom (AL+ECT). Polifenolni spojevi iz ekstrakta cvijeta Prunus spinosa L., u homogenatima jetre i bubrega, su statistički značajno smanjili aktivnost glukoze u krvi, inzulina i α-amilaze u skupini Al+ECT u odnosu na AL grupu. Statistički značajno smanjenje koncentracije AOPP u AL+ECT grupi ukazuje na antioksidacijski učinak ECT-a. Zaključno, ekstrakt cvijeta Prunus spinosa L. može pozitivno djelovati na hiperglikemiju i oksidacijski stres, te može poslužiti kao suportivna terapija u liječenju šećerne bolesti.

Abstract (english)

Numerous studies show that a diet rich with polyphenolic compounds helps regulate hyperglycemia. Prunus spinosa L. flower extract was observed due to its rich polyphenolic composition, and its effect on insulin levels, blood sugar levels, α-amylase, and oxidation protein products (AOPP) in C57BL/6 mice. Hyperglycaemia was induced in mice by alloxan (150 mg kgˉ¹ bw) and treated with Prunus spinosa L. flower extract at a dose of 25 mg kgˉ¹ bw with total polyphenols. The experiment lasted 10 days on mice divided into four groups. Group 1 as control (C), group 2 as Prunus spinosa L. flower extract (PSE), group 3 as aloxan (AL) and group 4 as AL with PSE. Polyphenolic compounds from Prunus spinosa L. flower extract in liver and kidney homogenates showed a statistically significant decrease in blood sugar levels, insulin and α-amylase activity in the group AL with PSE compared to the AL group. A statistically significant decrease in AOPP concentration in the group AL with PSE indicates the antioxidant effect of PSE. In conclusion, Prunus spinosa L. flower extract can have a positive effect on hyperglycemia and oxidative stress and can serve as supportive therapy in the treatment of diabetes.

• Keywords: trnina (Prunus spinosa L.); oksidacijski stres; hiperglikemija; AOPP; inzulin
• Keywords (english): blackthorn (Prunus spinosa L.); oxidative stress; hyperglycemia; AOPP; Insulin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:870065
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-16 09:53:06