In vitro ispitivanje toksičnosti kationiziranih i antimikrobno obrađenih tekstilija

master's thesis

• Title (english): In vitro toxicity assessment of cationized and antimicrobic treated textiles
• Author: Doroteja Prlok
• Mentor: Teuta Murati (mentor)
• Committee member: Ivana Kmetič (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2020-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Prisutnost određenih mikroorganizama na tekstilijama može imati brojne negativne posljedice za čovjeka i tekstilne proizvode. Zbog sve veće svijesti o patogenim učincima i pridruženim zdravstvenim rizicima, u posljednjih nekoliko godina provode se istraživanja kako bi se smanjio rast mikroorganizama na tekstilijama. Stoga se, u svrhu kontrole razvoja i razmnožavanja mikroorganizama, provode antimikrobne obrade tekstilija. Antimikrobna obrada tekstilija mora biti djelotvorna na široki spektar različitih vrsta gljiva i bakterija uz istovremenu neškodljivost za ljudsko zdravlje i okoliš. U ovom radu je ispitana potencijalna toksičnost tvari kojima su impregnirane tekstilije namijenjene upotrebi u bolnicama na staničnoj liniji humanih keratinocita, HaCaT. Za praćenje stanične proliferacije i vijabilnosti korištena je MTT metoda nakon 48 h i 72 h. Primijećeno je smanjenje stanične vijabilnosti i proliferacije kod svih ispitanih uzoraka tretiranih različitim razrijeđenjima ishodne otopine dobivene ekstrakcijom pamuk i pamuk/poliester tekstilija.

Abstract (english)

The growth of certain microorganisms on textiles can have a number of negative consequences for humans and textile itselfs. Due to the growing awareness of the pathogenic effects and associated health risks, over the last few years, intensive researchhas been conducted in order to reduce the growth of microorganisms on textiles. Therefore, in order to control the development and reproduction of microorganisms, antimicrobial textile treatments are carried out. Antimicrobial treatment of textiles should be effective against a broad spectrum of fungal and bacterial species but at the same time harmless to human health and the environment. In this study, the potential toxicity of textile impregnated substances for use in hospitals has been investigated using human keratinocyte cell line HaCaT. The MTT method after 48 h and 72 h was used to determine cell proliferation and viability. A decrease in cell viability and proliferation was observed in all tested samples treated with different dilutions of solution obtained by extraction of cotton and cotton/polyester textiles.

• Keywords: antimikrobna svojstva tekstilija; HaCaT; kitozan; MTT metoda; vijabilnost
• Keywords (english): antimicrobial properties of textiles; HaCaT; chitosan; MTT method; viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:109456
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-20 09:41:23