Usporedba bioraspoloživosti aktivnih tvari iz tekućih i mikroinkapsuliranih ekstrakata ljekovitog mediteranskog bilja

master's thesis

• Title (english): Comparison of bioavailability of active substances from liquid and microencapsulated extracts of medicinal Mediterranean herbs
• Author: Ivana Sarić
• Mentor: Maja Benković (mentor)
• Committee member: Jasenka Gajdoš Kljusurić (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Section for Fundamental Engineering); Zagreb
• Defense date and country: 2020-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Mediteranska se dijeta pokazala kao jedna od najuspješnijih u prevenciji kardiovaskularnih bolesti. Osim primjene maslinova ulja u prehrani, njena je posebnost i u upotrebi ljekovitog bilja u obliku začina ili čaja. Stoga su, u okviru ovog rada, ispitana svojstva pet biljnih uzoraka karakterističnih za mediteransko podneblje: matičnjaka, metvice, majčine dušice, kadulje i lavande. Ekstrakcijom pomoću vode te adsorpcijom na alginat dobiveni su tekući, odnosno mikroinkapsulirani ekstrakti. Proizvedenim ekstraktima određivana su fizikalna (pH, ukupne otopljene tvari, vodljivost, suha tvar) i kemijska svojstva (udio polifenola i antioksidacijska aktivnost). Kod obje se vrste ekstrakta (tekućeg i inkapsuliranog) matičnjak pokazao kao uzorak s najvišom koncentracijom polifenola te, prema DPPH i FRAP metodi, najvećom antioksidacijskom aktivnosti. Najmanje vrijednosti za navedene parametre imali su ekstrakti lavande. Analizom otpuštanja bioaktivnih tvari iz mikrokapsula utvrđeno je da se najveća količina bioaktivnih tvari otpustila iz mikrokapsula matičnjaka. Analizom kemijskih svojstava ekstrakata nakon in vitro probave, utvrđen je znatan pad koncentracije polifenola i antioksidacijske aktivnosti kod tekućih uzoraka, dok su mikroinkapsulirani ekstrakti pokazali veći stupanj očuvanja.

Abstract (english)

The Mediterranean diet has proven to be among the most efficient ones in preventing cardiovascular diseases. In addition to the use of olive oil in the diet, its distinguishing trait is the use of medicinal herbs in form of spices or tea. Therefore, this paper examines the properties of five Mediterranean plant samples: lemon balm, mint, thyme, sage and lavender. Liquid and encapsulated extracts had been prepared by extraction with water and adsorption by alginate. Physical (pH, total dissolved solids, conductivity, dry matter) and chemical properties (TPC and antioxidant activity) were then determined for the produced extracts. Within both types of extract (liquid and microencapsulated), lemon balm proved to be the sample with the highest concentration of polyphenols and, according to the DPPH and FRAP methods, the one with the highest antioxidant activity. The lowest values for the stated parameters were shown by the lavender samples. Similar results were obtained by the release test, where the release of active substances from microcapsules was observed. Chemical analysis of the extracts after in vitro digestion revealed a significant decrease in polyphenol concentration and antioxidant activity in liquid samples, while microencapsulated extracts showed a higher survival rate.

• Keywords: mediteransko bilje; mikroinkapsulacija; polifenoli; antioksidacijska aktivnost; in vitro probava
• Keywords (english): Mediterranean plants; polyphenols; microencapsulation; antioxidant activity; in vitro digestion
• Language: croatian
• URN:NBN: urn:nbn:hr:159:050594
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-20 11:58:13