Utjecaj odabranih sojeva bakterija mliječne kiseline na dentalne patogene

undergraduate thesis

• Title (english): Influence of selected strains of lactic acid bacteria on dental pathogens
• Author: Mislav Sušac
• Mentor: Jadranka Frece (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2020-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Prevencija i zaštita od različitih dentooralnih oboljenja važan je čimbenik s direktim utjecajem na kvalitetu života. Nažalost, unatoč konstantnim naporima u Republici Hrvatskoj trend kvalitete dentooralnog zdravlja je negativan te je pojavnost srodnih oboljenja u porastu. Navednom problemu se pristupa liječenjem, ali i sustavnom prevencijom koja je navedena kao jedan od ciljeva nacionalnog programa za preventivu i zaštitu oralnog zdravlja. U pristupu navedenim problemima ističe se pravilna higijena kako bi se smanjila pojavnost patogenih mikroorganizama, uzročnika dentooralnih oboljenja. Novija istraživanja ukazuju na djelotvornost probiotičkih pripravaka u prevenciji i redukciji spomenutih oboljenja. Cilj ovoga rada bilo je kvantificirati sposobnost formiranja biofilmova odabranih patogena uzročnika dentooralnih oboljenja i odabranih sojeva bakterija mliječne kiseline, potencijanih probiotika te odrediti antimikrobni utjecaj istraživanih bakterija mliječne kiseline na patogene mikroorganizme. Svi testirani patogeni umjereni su do jaki producenti biofilma, dok je osam od devet bakterija mliječne kiseline također klasificirano kao jaki producenti biofilma. Većina potencijalnih probiotičkih bakterija pokazala je značajnu antimikrobnu aktivnost prema testiranim patogenima.

Abstract (english)

Prevention and protection against various dental and oral diseases is an important factor with a direct impact on quality of life. Unfortunately, despite constant efforts in the Republic of Croatia, the trend in the quality of dental health is negative and the incidence of related diseases is increasing. This problem is approached through treatment, but also systemic prevention, which is listed as one of the goals of the national program for prevention and protection of oral health. In the approach to these problems, proper hygiene is emphasized in order to reduce the occurrence of pathogenic microorganisms, the most common causes of dental and oral diseases. Recent research indicates the effectiveness of probiotic preparations in the prevention and reduction of these diseases. The aim of this study was to quantify the ability to form biofilms of selected pathogens of dental and oral diseases and selected strains of lactic acid bacteria, potential probiotics, and to determine the antimicrobial effect of the studied lactic acid bacteria on pathogenic microorganisms. All pathogens tested were moderate to strong biofilm producers, while eight of the nine lactic acid bacteria were also classified as strong biofilm producers. Most potential probiotic bacteria showed significant antimicrobial activity against the tested pathogens.

• Keywords: antimikrobno; biofilm; dentalni patogeni; probiotici
• Keywords (english): antimicrobial; biofilm; dental pathogens; probiotics
• Language: croatian
• URN:NBN: urn:nbn:hr:159:471484
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-06 11:17:00