Karakterizacija probiotičke bakterije Lactobacillus plantarum O1 za biološko konzerviranje proizvoda akvakulture

doctoral thesis

• Title (english): Characterization of probiotic bacterium Lactobacillus plantarum O1 for biological conservation of aquaculture products
• Author: Iva Čanak
• Mentor: Jadranka Frece (mentor)
• Committee member: Ksenija Markov (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2020-12-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 638/639 - Apiculture. Keeping, breeding and management of insects and other arthropods. Hunting. Fishing. Fish breeding

Abstract

Cilj ovog rada, uz polazno ljetno i zimsko utvrđivanje prisutnosti mikroorganizama odgovornih za zdravstvenu ispravnost ribe i školjkaša, bio je iz izolata autohtone mikroflore probavnog sustava te sluzi kože i škrga ribe i školjkaša, izolirati, identificirati i karakterizirati prisutne bakterije mliječne kiseline (BMK) te odabrati onu s najvećim konzervirajućim potencijalom. Izolat autohtone mikroflore pripremljen je od komercijalno najzastupljenijih vrsta u hrvatskoj marikulturi-lubina (Dicentrarchus labrax), orade (Sparus aurata), dagnji (Mytilus galloprovincialis) i kamenica (Ostrea edulis). Utvrđena je prisutnost pet sojeva BMK: Lactobacillus plantarum O1, L. plantarum O9, Leuconostoc mesenteroides L4A, L. plantarum K4 i L. plantarum D1. Potom je obavljena karakterizacija svake pojedinačne izolirane BMK kulture sukladno selekcijskim kriterijima za odabir BMK za primjenu u akvakulturi. Rezultati ovog rada upućuju na veliki potencijal primjene BMK morskog porijekla u akvakulturi-kao probiotika s ciljem preveniranja i smanjivanja bolesti morskih životinja ili kao bioloških konzervansa s ciljem dobivanja novih vrsta ekološki održivih proizvoda prirodno produljenog roka trajnosti.

Abstract (english)

The aim of this study, in addition to the initial summer and winter determination of the presence of microorganisms responsible for the health of fish and shellfish, was to isolate, identify and characterize the lactic acid bacteria (LAB) present in isolates of the autochthonous microflora of the digestive system, skin mucus and gills of fish and shellfish, and choose the one with the greatest preservative potential. The isolate of autochthonous microflora was prepared from the most commercially represented species in Croatian mariculture - sea bass (Dicentrarchus labrax), sea bream (Sparus aurata), mussels (Mytilus galloprovincialis) and oysters (Ostrea edulis). The presence of five LAB strains was determined: Lactobacillus plantarum O1, L. plantarum O9, Leuconostoc mesenteroides L4A, L. plantarum K4 and L. plantarum D1. The characterization of each individual isolated pure LAB culture was then performed according to the selection criteria for the selection of LAB for use in aquaculture. The results of this work point the great potential of marine origin LAB in aquaculture - as a probiotic to prevent and reduce diseases of marine animals or as biological preservatives to obtain new types of environmentally sustainable products with naturally extended shelf life.

• Keywords: akvakultura; Lactobacillus plantarum; mikrobiota; probiotici
• Keywords (english): aquaculture; Lactobacillus plantarum; microbiota; probiotics
• Language: croatian
• URN:NBN: urn:nbn:hr:159:119103
• Study programme: Title: Food Technology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• Extent: 107 str. : 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-28 14:22:57