Application of natural deep eutectic solvents in isolation of anthocyanins and biocatalysis with lipase

doctoral thesis

• Title (croatian): Primjena prirodnih eutektičkih otapala u izolaciji antocijana i biokatalizi pomoću lipaze
• Author: Manuela Panić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Committee member: Marina Cvjetko Bubalo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-03-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

In this dissertation, 15 natural deep eutectic solvents were successfully prepared and applied for developing an eco-friendly method for extraction of anthocyanins and lipase-catalyzed (R)-1-phenylethanol synthesis. Also, the biological activity of prepared grape pomace extracts was evaluated. With natural deep eutectic solvents efficiency of the process was increased in comparison with conventional processes. Extraction and biocatalytic process were optimised using the response surface methodology afterward laboratory-developed methods were scaled up. In both processes, the recyclation of used natural deep eutectic solvents was performed, as well as isolation and purification of the final product (anthocyanins, (R)-1-phenylethanol).

Abstract (croatian)

U sklopu doktorske disertacije uspješno je pripremljeno i okarakterizirano 15 prirodnih eutektičkih otapala i primijenjeno za razvoj ekološki prihvatljivih metoda ekstrakcije antocijana iz komine grožđa te biokatalitičkog procesa priprave (R)-1-feniletanola pomoću lipaze. Također, evaluirana je biološka aktivnost pripremljenih polifenolnih ekstrakata u prirodnim eutektičkim otapalima. Primjenom prirodnih eutektičkih otapala u ekstrakciji polifenolnih spojeva i biokatalizi ostvarena je veća učinkovitost procesa u odnosu na konvencionalne postupke. Ekstrakcija i biokataliza su optimirani primjenom metode odzivnih površina te su laboratorijski razvijene metoda prenijete u veće mjerilo. U oba procesa razvijena je i metoda reciklacije korištenog prirodnog eutektičkog otapala, kao i izolacije i pročišćavanja produkta (antocijani, (R)-1-feniletanol).

• Keywords: (R)-1-phenylethanol; biological activitiy; grape pomace; green chemistry; green extraction; natural deep eutectic solvents; Novozyme 435; polyphenolic compounds; solvent recycling; stereoselective biocatalysis
• Keywords (croatian): (R)-1-feniletanol; biološka aktivnost; komina grožđa; Novozyme 435; polifenolni spojevi; prirodna eutektička otapala; reciklacija otapala; stereoselektivna biokataliza; zelena ekstracija; zelena kemija
• Language: english
• URN:NBN: urn:nbn:hr:159:069138
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
• Type of resource: Text
• Extent: 113 str. : 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-29 14:01:35