Genotoksikološki profil površinskih, podzemnih i procjednih voda s područja odlagališta otpada Piškornica

master's thesis

• Title (english): Genotoxicological profile of surface water, groundwater and leachate from the area of the waste landfill Piškornica
• Author: Adriana Ćurić
• Mentor: Ksenija Durgo (mentor)
• Committee member: Anamarija Štafa (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2021-01-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Svijet je suočen s nedostatkom zdravstveno ispravne vode za piće prvenstveno radi ugrožavanja vodenih okoliša pri čemu je jedan od glavnih izvora onečišćenja nepravilno gospodarenje otpadom. Danas je najčešća metoda zbrinjavanja otpada odlaganje razvrstanog otpada na za to predviđena mjesta koja se redovito moraju kontrolirati jer vrlo lako može doći do procjeđivanja raznih zagađivača u podzemne i površinske vode. Cilj ovog istraživanja bio je odrediti citotoksičnost, genotoksičnost te oksidacijsko oštećenje staničnih makromolekula proteina i lipida uzrokovanog djelovanjem voda s područja odlagališta otpada Piškornica. Kao biološki test sustav korištene su humane stanične linije hepatocelularnog karcinoma jetre Hep G2 te oralnog pločastog karcinoma Cal 27, a vrijeme izloženosti bilo je 24 sata. Rezultati su pokazali da uzorak procjedne vode djeluje citotoksično te izaziva karbonilaciju proteina i lipidnu peroksidaciju. Zbog smanjenog postotka preživljenja (<75 %) genotoksični učinak za uzorak procjedne vode nije bilo moguće odrediti. Izuzev uzoraka podzemne vode koji su izazvali lipidnu peroksidaciju, ostali ispitivani uzorci nisu djelovali citotoksično ni genotoksično, niti su izazvali oštećenje staničnih makromolekula (proteina, lipida i DNA).

Abstract (english)

The world has been facing with the major lack of safe and readily available drinking water because of the endangerment of the aquatic envirnoment mostly caused by inappropriate waste management. Commonly used method is waste disposal at landfills which, under certain circumstances, can cause contamination of surface water or groundwater. The aim of this research is to determine potential citotoxic and genotoxic effect and to determine possible damage of cellular proteins and lipids caused by different types of water from the area of the waste landfill Piškornica. As the test system, a human hepatocellular carcinoma cell line Hep G2 and oral squamous carcinoma cell line Cal 27 was used, and it was exposed to the examined samples of water for 24 hours. The results have shown that leachate sample has cytotoxic effect and causes protein carbonylation and lipid peroxidation. As in the case of leachate sample the cell viability didn't exceed 75 % and the genotoxic effect couldn't be determined. While the groundwater samples caused increased lipid peroxidation, other samples didn't have cytotoxic nor genotoxic effect or caused the damage of cellular macromolecules (proteins, lipids and DNA).

• Keywords: odlagalište Piškornica; voda; genotoksičnost; citotoksičnost; testovi in vitro
• Keywords (english): landfill Piškornica; water; genotoxicity; cytotoxicity; in vitro tests
• Language: croatian
• URN:NBN: urn:nbn:hr:159:023343
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-17 11:37:06