Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae

undergraduate thesis

• Title (english): Interplay of mRNA-binding protein Ssd1 and 3'-5' exoribonuclease Rrp6 in yeast cell wall stability maintenance
• Author: Nikša Bekavac
• Mentor: Igor Stuparević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2021-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

RNA-vezujući protein Ssd1 regulira ekspresiju gena koji kodiraju za proteine uključene u stabilizaciju stanične stijenke kvasca. U očuvanju integriteta stanične stijenke bitnu ulogu ima i RNA egzosom, kompleks koji procesira i degradira RNA supstrate egzoribonukleaznom aktivnošću katalitičkih podjedinica Dis3 i Rrp6. Stanice mutanata rrp6 pokazuju temperaturnu osjetljivost koja je posljedica kompromitirane stabilnosti stanične stijenke, a ovaj fenotip je najizraženiji kod linije sojeva kvasca W303. Budući da sojevi ove linije nose nefunkcionalnu inačicu alela koji kodira za Ssd1 (ssd1-d), ispitan je utjecaj ekspresije funkcionalnog alela SSD1-V s odgovarajućeg genomskog lokusa i centromernog plazmida u stanicama mutanata W303 rrp6 i dis3 exo-. Ekspresija SSD1-V suprimirala je defekte u rastu i morfologiji stanica ovih mutanata u odnosu na odgovarajuće ssd1-d stanice pri djelovanju povišene temperature i agenasa koji oštećuju staničnu stijenku. Ovi rezultati upućuju da RNA egzosom i Ssd1 djeluju sinergistički pri održavanju integriteta stanične stijenke kvasca.

Abstract (english)

The RNA-binding protein Ssd1 regulates the expression of genes encoding yeast cell wall morphogenesis proteins. Maintenance of yeast cell wall integrity is also mediated by the RNA exosome, a complex which processes and degrades RNA substrates through exoribonuclease activities of its catalytic subunits, Dis3 and Rrp6. Yeast rrp6 mutant cells display a temperature-sensitive phenotype as a result of impaired cell wall stability, which is most pronounced in the W303 genetic background. Since strains of this background harbour a non-functional form of the allele encoding Ssd1 (ssd1-d), in this work we tested the effect of the expression of the active SSD1-V allele from its corresponding genomic locus and from a centromeric plasmid in W303 rrp6 and dis3 exo- mutant cells. Expression of SSD1-V suppressed growth and morphology defects upon exposure to high temperature and cell wall stressors compared with the corresponding ssd1- d cells. These results suggest that RNA exosome and Ssd1 act synergistically during the maintenance of cell wall integrity in yeast.

• Keywords: kvasac Saccharomyces cerevisiae; RNA egzosom; Rrp6; Ssd1; stanična stijenka
• Keywords (english): cell wall; RNA exosome; Rrp6; Ssd1; yeast Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:834967
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-06-18 09:36:11