Održivost crijevne mikrobiote u in vitro uvjetima

master's thesis

• Title (english): Viability of intestinal microbiota during in vitro cultivation
• Author: Elena Malešić
• Mentor: Antonio Starčević (mentor)
• Committee member: Blaženka Kos (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Crijevna mikrobiota u posljednje vrijeme sve je više fokus istraživanja te joj se pripisuju brojni pozitivni učinci na zdravlje čovjeka. Osim toga, sve se više povezuje mikrobna populacija donjeg dijela gastrointestinalnog trakta s etiologijom mnogih bolesti poput infekcije Clostridium difficile, upalnih bolesti crijeva i pretilosti. U svrhu liječenja takvih patoloških stanja, u novije vrijeme koristi se i fekalna mikrobna transplantacija kao alternativa klasičnim metodama liječenja. S obzirom na njene brojne nedostatke, cilj ovog rada je bio uzgojiti crijevnu mikrobiotu u uvjetima in vitro te ju usporediti s onom u nativnom fecesu iz kojeg potječe (inokulumu), kako bi se ustvrdilo je li moguće u takvim uvjetima dobiti stabilnu zajednicu mikroorganizama dovoljno visoke raznolikosti. Alfa i beta raznolikost te taksonomski profil crijevne mikrobiote uzoraka određeni su 16S rRNA sekvenciranjem te obradom podataka u QIIME 2 programu. Prema rezultatima ovog rada, uočena je smanjena bakterijska raznolikost uzoraka fecesa uzgojenih u bioreaktoru te promjena u taksonomskoj zastupljenosti bakterijskih vrsta u usporedbi s inokulumom. Unatoč tome, in vitro kultivacija uzoraka fecesa bi mogla poslužiti kao temelj za liječenje fekalnom mikrobnom transplantacijom uz prijeko potrebna dodatna istraživanja.

Abstract (english)

The intestinal microbiota has recently become the focus of research and is credited with numerous positive effects on human health. In addition, the microbial population of the lower gastrointestinal tract is increasingly associated with the etiology of various diseases such as Clostridium difficile infection, inflammatory bowel disease and obesity. To treat such pathological conditions, fecal microbial transplantation has recently been used as an alternative to already established methods of treatment. Given the numerous shortcomings of fecal transplantation, the aim of this study was to cultivate the intestinal microbiota in vitro and compare it with the native feces from which it originates (inoculum) in order to determine whether it is possible to obtain a stable and sufficiently diverse community of microorganisms in such controlled conditions. Alpha and beta diversity as well as the taxonomic profile of the intestinal microbiota of the samples were determined by 16S rRNA sequencing and data processing was performed using the QIIME 2 program. According to the results of this work, a decrease in the bacterial diversity of fecal samples cultivated in the bioreactor and changes in the taxonomic representation of bacterial species compared to the inoculum were observed. Nevertheless, in vitro cultivation of fecal samples could serve as a basis for fecal microbial transplantation treatment with additional research required.

• Keywords: crijevna mikrobiota; in vitro uzgoj; fekalna transplantacija; 16S rRNA sekvenciranje; QIIME 2
• Keywords (english): gut microbiota; in vitro cultivation; fecal transplantation; 16S rRNA sequencing; QIIME 2
• Language: croatian
• URN:NBN: urn:nbn:hr:159:587792
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-25 10:17:27