Proteini staničnih stijenki različitih rodova kvasaca i njihov potencijal za izlaganje heterolognih proteina na staničnoj površini

doctoral thesis

• Title (english): Cell wall proteins of yeasts of different genera and their potential for cell surface display of heterologous proteins
• Author: Mateja Lozančić
• Mentor: Vladimir Mrša (mentor)
• Committee member: Renata Teparić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2021-05-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Stanična stijenka obavija stanice kvasaca dajući im oblik i zaštitu od nepovoljnih okolišnih uvjeta, a sastoji se od β-glukana, hitina i manoproteina. Ima i biotehnološki potencijal te se tako između ostaloga koristi i za izlaganje proteina od interesa na staničnoj površini. Trenutno razvijeni sustavi izlaganja proteina imaju brojna ograničenja koja je moguće prevladati razvojem novih sustava te pronalaskom novih stanica domaćina takvih sustava. U svrhu traženja novih potencijalnih vrsta kvasaca za korištenje u spomenute svrhe, potrebno je generirati znanje o postojećim proteinima stijenki tih kvasaca. U ovoj studiji provest će se in silico analiza proteina koji sudjeluju u izgradnji stijenke ili su u njoj lokalizirani na temelju usporedbe proteoma kvasaca iz različitih rodova s proteomom stanične stijenke kvasca Saccharomyces cerevisiae, a analiza će biti nadopunjena usporedbom proteinskih profila stijenki kvasaca. Također, ispitivat će se mogućnost primjene razvijenih sustava za izlaganje proteina za kvasac S. cerevisiae u kvascu Komagataella phaffi. U konačnici, ispitivat će se način kovalentne ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca u svrhu mogućeg korištenja ovih proteina za razvoj novih sustava izlaganja proteina na površini stanica.

Abstract (english)

Yeast cells are surrounded by a cell wall that determines cell shape and provides protection from stress environmental conditions. It is composed of β-glucan, chitin, and mannoproteins. The cell wall is of biotechnological interest as it can be used for the surface display of proteins of interest. Currently developed surface display systems have many limitations that could be overcome with the development of novel surface display systems and as well with finding new hosts for these systems. To find suitable host yeast strains, knowledge about proteins located in their walls should be generated. To do so, comparative in silico analysis of yeast cell wall proteome of Saccharomyces cerevisiae and proteomes of the yeast of different genera will be performed as a part of this thesis and coupled with analysis of cell wall protein profiles. Also, the applicability of surface display systems developed for S. cerevisiae for the display of proteins in Komagataella phaffii will be tested. Finally, covalent incorporation of S. cerevisiae cell wall proteins Scw4 and Scw10 will be analyzed for purpose of applying these proteins in novel surface display system development.

• Keywords: proteom stanične stijenke; proteinski profili stijenke; izlaganje proteina na staničnoj površini; Komagataella phaffii; Scw4 i Scw10
• Keywords (english): yeast cell wall proteom; cell wall protein profiles; surface display systems; Komagataella phaffii; Scw4 and Scw10
• Language: croatian
• URN:NBN: urn:nbn:hr:159:821458
• Promotion: 2021
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
• Type of resource: Text
• Extent: 146 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-23 13:32:58