Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma

master's thesis

• Title (english): Targeted imaging probes for Netrin-1 - a novel metastatic breast cancer biomarker: small molecules to liposomes
• Author: Tea Lukačević
• Mentor: Vladimir Mrša (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2021-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Despite significant development of cancer imaging and treatment over the years, the challenge of early cancer diagnosis and detection of metastasis remains. Molecular imaging probes for magnetic resonance imaging (MRI, a non-invasive clinically used imaging technique with high resolution) are well adapted to solve this problem, namely via the design of new contrast agents, which target specific tumor biomarkers. The protein Netrin-1 is an extracellular protein involved in regulation of cell migration and apoptosis. This study focuses on the evaluation of Netrin-1 as a potential biomarker in metastatic breast cancer imaging. Two types of contrast agents targeted to Netrin-1 are explored: small molecule-based probes and nanoparticles (liposomes). The designed MRI probes were examined using in vitro binding assays and measuring relaxivities to assess their potential MRI application. Herein we have shown successful selectivity of both types of probes towards Netrin-1 and significant increase in relaxivity values, especially in case of nanoparticles.

Abstract (croatian)

Unatoč značajnim naprecima u dijagnostici i liječenju raka tijekom posljednjih godina, rano otkrivanje raka i detekcija metastaza predstavlja nepremošćen izazov. Molekularne detekcijske probe za magnetsku rezonancu (MR, ne invazivna klinička dijagnostička metoda visoke rezolucije) dobri su kandidati za rješavanje ovog problema, posebice kao osnova za razvijanje novih kontrasta specifičnih za određene tumorske biomarkere. Protein Netrin-1 je izvanstanični protein koji je uključen u procese migracije stanica i apoptoze. Provedeno istraživanje usmjereno je na ispitivanje potencijala proteina Netrin-1 kao mogućeg biomarkera u dijagnostici metastatskog karcinoma dojke. Korištene su dvije vrste kontrastnih agenata specifičnih za protein Netrin-1 : male molekularne probe i nanočestice (liposomi). Razvijene MR probe su ispitane koristeći in vitro testove afinitetnog vezivanja te mjerenjem relaksivnosti kako bi se ocijenila uspješnost njihove moguće primjene u MR dijagnostici. U ovom radu prikazana su uspješno uspostavljena specifičnost obje vrste proba prema proteinu Netrin-1 te značajna povećanja u vrijednostima relaksivnosti, posebice u slučaju nanočestica.

• Keywords: metastasis; magnetic resonance imaging; contrast agents; biomarkers; Netrin-1
• Keywords (english): metastaze; magnetska rezonanca; kontrasti; biomarkeri; Netrin-1
• Language: english
• URN:NBN: urn:nbn:hr:159:840515
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-05-26 11:28:24