Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka

doctoral thesis

• Title (english): Determination of nonsteroidal anti-inflammatory drug residues and in vitro assessment of their ecotoxicological impact
• Author: Đurđica Božić Luburić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Mentor: Nina Bilandžić (komentor)
• Committee member: Kristina Radošević (predsjednik povjerenstva)
• Committee member: Višnja Gaurina-Srček (član povjerenstva)
• Committee member: Ivana Varenina (član povjerenstva)
• Committee member: Ivana Kmetič (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2022-03-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

U okviru doktorske disertacije razvijena je metoda za kvantitativno određivanje ostataka 27 nesteroidnih protuupalnih lijekova, kemijski različitih spojeva, tekućinskom kromatografijom ultra visoke djelotvornosti povezane s masenom spektrometrijom u uzorcima hrane životinjskog podrijetla (mlijeku, mišićnom tkivu) i uzorcima vode iz okoliša (otpadna voda). Optimiziran postupak ekstrakcije iz složenih matrica temelji se na enzimskoj hidrolizi koju slijedi ekstrakcija acetonitrilom, a dobiveni ekstrakt je pročišćen, uz istovremeno koncentriranje, ekstrakcijom na čvrstoj fazi. Validacijom metode ispitane su zadane značajke učinkovitosti koje su zadovoljile zakonski postavljene kriterije prihvatljivosti. Istraživanje je obuhvatilo i analizu realnih uzoraka s područja Republike Hrvatske, 449 uzoraka hrane životinjskog podrijetla i 96 uzoraka otpadnih voda. Kako bi se dobile informacije o toksičnosti tih spojeva koji dospijevaju u okoliš, in vitro testom na staničnim linijama ribe i ljudi ispitan je njihov citotoksični učinak.

Abstract (english)

In this dissertation, a method for the quantitative determination of 27 nonsteroidal anti-inflammatory drug residues by ultra high performance liquid chromatography – mass spectrometry in food of animal origin (milk, muscle) and environmental water (wastewater) was developed. Optimised sample processing consisted of enzymatic hydrolysis and acetonitrile extraction was followed by a clean-up of the extract by solid phase extraction. By validation of the method, verified performance characteristics complied with the legally set acceptability criteria. The study also included the analysis of the real samples from regions of the Republic of Croatia, 449 samples of food of animal origin and 96 samples of wastewater. In order to obtain information on the toxicity of these compounds that are released into the environment, their cytotoxic effect was examined using in vitro test on fish and human cell lines.

• Keywords: nesteroidni protuupalni lijekovi; ostaci (rezidue); UHPLC-MS/MS; validacija; ekotoksikologija; hrana životinjskog podrijetla; otpadne vode
• Keywords (english): nonsteroidal anti-inflammatory drugs; residues; UHPLC-MS/MS; validation; ecotoxicology; food of animal origin; wastewater
• Language: croatian
• URN:NBN: urn:nbn:hr:159:409424
• Promotion: 2022
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
• Type of resource: Text
• Extent: 133 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-09 12:46:17