Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26

master's thesis

• Title (english): The role of Rab protiens in the adenovitus type 26 transduction
• Author: Klara Kašnar
• Mentor: Ksenija Durgo (mentor)
• Committee member: Marina Cvjetko Bubalo (predsjednik povjerenstva)
• Committee member: Dragomira Majhen (član povjerenstva)
• Committee member: Igor Stuparević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2022-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Rekombinantni adenovirusni vektori koriste se kao viralni vektori u genskoj terapiji i vakcinaciji. Najčešće korišten adenovirusni vektor temeljen je na adenovirusu tipa 5 (HAdV-C5) čija je uporaba ograničena zbog visoke seroprevalencije pa se sve više istražuju manje seroprevalentni adenovirusi kao što je adenovirus tipa 26 (HAdV-D26). Rab proteini imaju brojne uloge u stanici, a smatraju se ključnim regulatorima staničnog transporta. U ovom radu ispitana je uloga Rab5, Rab7, Rab9 i Rab11 proteina u endocitozi i unutarstaničnom putovanju HAdV-D26. Stanice su transficirane plazmidima koji sadrže gene za Rab proteine divljeg tipa (WT, engl. wild type) te je uspješnost transdukcije tih stanica uspoređena s uspješnošću transdukcije stanica transficiranih plazmidima koji sadrže gene za dominantno negativne (DN, engl. dominant negative) mutante Rab proteina. Zabilježena je manja uspješnost transdukcije u stanicama transficiranima s plazmidima koji kodiraju za DN mutante Rab proteina što ukazuje na ulogu Rab proteina u endocitozi i unutarstaničnom putovanju HAdV-D26.

Abstract (english)

Recombinant adenoviral vectors are used as viral vectors in gene therapy and vaccination. The most used adenovirus vector is based on adenovirus type 5 (HAdV-C5), but its use is limited due to the high seroprevalence thus, less seroprevalent adenoviruses such as adenovirus type 26 (HAdV-D26) are investigated as alternative. Rab proteins have numerous roles in the cell and are considered key regulators of cellular transport. In this study we investigatedthe role of Rab5, Rab7, Rab9 and Rab11 proteins in endocytosis and intracellular trafficking of HAdV-D26. HAdV-D26 transduction was compared in cells transfected with plasmids containing genes for wild-type (WT) Rab proteins and cells transfected with plasmids containing genes for dominant negative (DN) mutants of Rab proteins. A lower transduction efficiency was observed in cells transfected with plasmids encoding dominant negative mutants indicating the role of Rab proteins in endocytosis and intracellular trafficking of HAdV-D26.

• Keywords: adenovirus čovjeka tipa 26; Rab proteini; unutarstanično putovanje virusa
• Keywords (english): human adenovirus type 26; Rab proteins; intracellular trafficking of viruses
• Language: croatian
• URN:NBN: urn:nbn:hr:159:754444
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-13 14:44:15