Karakterizacija i genetička transformacija odabranih vrsta nekonvencionalnih kvasaca iz roda Schwanniomyces

master's thesis

• Title (english): Characterization and genetic transformation of selected species of unconventional yeasts from the genus Schwanniomyces
• Author: Ana Slišković
• Mentor: Marina Svetec Miklenić (mentor)
• Committee member: Igor Slivac (predsjednik povjerenstva)
• Committee member: Ivan Krešimir Svetec (član povjerenstva)
• Committee member: Renata Teparić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2022-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kako bi se ostvarila maksimalna efikasnost industrijskog bioprocesa, poželjno je da radni organizam bude otporan na širok raspon parametara procesnih uvjeta te da efikasno proizvodi željeni proizvod. Nekonvencionalni kvasci predstavljaju raznoliku skupinu organizama od kojih pojedine vrste prirodno posjeduju razne biotehnološki interesantne karakteristike te se sve češće koriste u proizvodnji. U skupinu nekonvencionalnih kvasaca spadaju i vrste iz roda Schwanniomyces, koje osim mogućnosti rasta na različitim izvorima ugljika pokazuju i efikasnu sekreciju proteina velike molekulske mase u podlogu. Unatoč navedenim prednostima, mnoge vrste ovog roda još uvijek nisu detaljno istražene zbog nedostatka prikladnih metoda za genetičku transformaciju i ciljane genetičke modifikacije. Stoga je cilj ovog rada bio transformirati kvasce Schwanniomyces occidentalis var. occidentalis, Schwanniomyces occidentalis var. persoonii, Schwanniomyces capriottii i Schwanniomyces vanrijiae var. yarrowii, te ispitati aktivnost ishodišta replikacije iz različitih vrsta kvasaca korištenjem plazmidnih vektora.

Abstract (english)

In order to achieve the maximum efficiency of industrial bioprocesses, it is desirable for the working organism to be resistant to a wide range of process conditions and to efficiently produce the desired product. Non-conventional yeasts represent a diverse group of organisms, some species of which naturally possess various biotechnologically interesting characteristics and are increasingly used in production. The group of non-conventional yeasts also includes species from the genus Schwanniomyces, which, in addition to the ability to grow on different carbon sources, also show efficient secretion of high molecular weight proteins into the substrate. Despite the mentioned advantages, many species of this genus have not yet been investigated in detail due to the lack of suitable methods for genetic transformation and targeted genetic modification. Therefore, the aim of this work was to transform the yeast Schwanniomyces occidentalis var. occidentalis, Schwanniomyces occidentalis var. persoonii, Schwanniomyces capriottii and Schwanniomyces vanrijiae var. yarrowii, and to examine the activity of origins of replication from different yeast species using plasmid vectors.

• Keywords: genetička transformacija; nekonvencionalni kvasci; rod Schwanniomyces; plazmidi
• Keywords (english): genetic transformation; unconventional yeasts; genus Schwanniomyces; plasmid vectors
• Language: croatian
• URN:NBN: urn:nbn:hr:159:072363
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-28 13:26:26