Optimization of extraction and encapsulation techniques of bioactive molecules from nettle (Urtica dioica L.)

doctoral thesis

• Title (croatian): Optimiranje postupaka ekstrakcije i inkapsulacije bioaktivnih molekula koprive (Urtica dioica L.)
• Author: Ena Cegledi
• Mentor: Verica Dragović-Uzelac (mentor)
• Committee member: Maja Repajić (predsjednik povjerenstva)
• Committee member: Sandra Balbino (član povjerenstva)
• Committee member: Sanja Radman (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2023, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology
• Universal decimal classification (UDC): 58 - Botany

Abstract

Nettle (Urtica dioica L.) is a plant rich in bioactive active molecules such as phenols, pigments and phytosterols, which have beneficial effects on human health and were the subject of this research. In order to isolate higher yields of mentioned bioactives from nettle, the parameters of accelerated solvent extraction were studied and optimized. In addition, the influence of phenological stage and habitat on the accumulation of bioactive molecules during the growing season was investigated. Furthermore, to protect and stabilize phenolics from nettle leaves, the parameters of encapsulation technique of spray drying were optimized, and it was determined that encapsulation had a positive effect on the bioavailability of phenolic compounds.

Abstract (english)

Kopriva (Urtica dioica L.) je biljka bogata biološki aktivnim molekulama poput fenola, pigmenata i fitosterola koji pozitivno utječu na zdravlje te su predmet ovog istraživanja. Za učinkovitu izolaciju spomenutih komponenata s maksimalnim prinosom iz ekstrakta koprive, ispitani su i optimirani parametri ubrzane ekstrakcije otapalima. Nadalje, ispitan je utjecaj fenotipske faze i staništa na akumulaciju biološki aktivnih molekula tijekom vegetacije. Nadalje, u svrhu zaštite i stabilizacije fenola iz lista koprive optimirani su parametri inkapsulacijske metode sušenja raspršivanjem te je utvrđeno kako inkapsulacija pozitivno utječe na bioraspoloživost fenolnih spojeva.

• Keywords: nettle; bioactive molecules; accelerated solvent extraction; spray drying
• Keywords (english): kopriva; bioaktivne molekule; ubrzana ekstrakcija otapalima; sušenje raspršivanjem
• Language: english
• URN:NBN: urn:nbn:hr:159:894962
• Promotion: 2023
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/ doktorica znanosti, područje biotehničkih znanosti, odgovarajuće polje (određeno matičnom strukom) (doktor/ doktorica znanosti, područje biotehničkih znanosti, odgovarajuće polje (određeno matičnom strukom))
• Type of resource: Text
• Extent: 139 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-27 08:47:53