Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima

master's thesis

• Title (english): Activity of lytic polysaccharide monooxygenase in low-temperature deep eutectic solvents
• Author: Ivan Perković
• Mentor: Tonči Rezić (mentor)
• Committee member: Marina Cvjetko Bubalo (predsjednik povjerenstva)
• Committee member: Ana Tušek Jurinjak (član povjerenstva)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-05-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ovim radom ispitana je aktivnost enzima litičke polisaharidne monooksigenaze (LPMO) u niskotemperaturnim eutektičkim otapalima (engl. Deep Eutectic Solvents, DES) te je izrađen jednostavan i robusan matematički model kojime se nastoji predviditi korištenje otapala s najprikladnijim fizikalno kemijskim svojstvima njegovih sastavnih komponenata za biokatalitičku reakciju LPMO. Odabrane su lako dostupne, netoksične i relativno jeftine komponente za izradu niskotemperaturnih eutektičkih otapala prema načelima zelene kemije i održivog razvoja. Nakon sinteze otapala, spektrofotometrijski je ispitana aktivnost LPMO prvotno u NaAc puferu pH 6,4 u svrhu usporedbe aktivnosti. Potom je aktivnost enzima ispitana u priređenim otapalima istom spektrofotometrijskom metodom uz pufer kao slijepu probu. Nadalje, DES-ovi su matematički opisani pomoću vrijednosti dobivenih COSMO-RS programskim paketom. Dobivene vrijednosti grupirane su u 10 intervala kako bi se načinio sveukupni sigma profil DES-a. Na osnovi izrađenih intervala programskim paketom Statistica 13.0 izrađeni su modeli višestruke linearne regresije te djelomične linearne regresije

Abstract (english)

This work investigated the activity of the lytic polysaccharide monooxygenase enyzme (LPMO) in low-temperature deep eutectic solvents (DES) and developed two simple and robust mathematical models to predict the use of solvents with the most suitable physicochemical properties of their components for the LPMO biocatalytic reaction. Easily accessible, non-toxic, and relatively inexpensive components were selected to create the deep eutectic solvents according to the principles of green chemistry and sustainable development. LPMO activity was initially spectrophotometrically tested in NaAc buffer pH 6,4 for comparison. Then, enzyme activity was tested in prepared solvents using the same method with buffer as a blank. Furthermore, DES were mathematically described using the COSMO-RS software package. The obtained values were grouped into 10 intervals to create a sigma profile of the solvents. Based on these intervals, multiple linear regression and piecewise linear regression models were developed using the Statistica 13.0 software package.

• Keywords: niskotemperaturna eutektička otapala; aktivnost; LPMO; COSMO-RS
• Keywords (english): low-temperature deep eutectic solvents; activity; LPMO; COSMO-RS
• Language: croatian
• URN:NBN: urn:nbn:hr:159:690731
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-02 09:00:13