Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama

master's thesis

• Title (english): Cultivation of mold Mucor indicus DSM 2185 under different aeration conditions and utilization of waste substrate by non-sulfur purple bacteria
• Author: Ivan Kovač
• Mentor: Mario Novak (mentor)
• Mentor: Nenad Marđetko (pomoć pri izradi, neposredni voditelj)
• Committee member: Antonija Trontel (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-07-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada bio je ispitati utjecaj različitih protoka zraka pri uzgoju plijesni Mucor indicus DSM 2185 na dva hidrolizata kukuruznih oklasaka u barbotirajućem bioreaktoru te istražiti mogućnost iskorištenja otpadnih prevrelih podloga za uzgoj fototrofnih nesumpornih ljubičastih bakterija. Uzgojem plijesni u 1 L podloge pri protoku zraka od 0,5 L/min na kiselinskom hidrolizatu proizvedeno je 5,07 g/L etanola i 5 g biomase s 8,3 % masnih kiselina, a na enzimskom hidrolizatu proizvedeno je 11,7 g/L etanola i 10,8 g biomase s 13,6 % masnih kiselina. Uzgoji provedeni pri 1 i 1,5 L/min zraka proizveli su više biomase s većim udjelom masnih kiselina s manje etanola u podlozi. U anaerobnim uvjetima proizvedeno je 22,35 g/L etanola. Na iskorištenim podlogama nakon uzgoja plijesni proveo se uzgoj bakterija Rhodovulum adriaticum DSM 2178 i za Rhodobacter capsulatus JCM 21090 te se pratila sinteza bakterioklorofila, te je napravljena analiza masnih kiselina u izoliranoj biomasi.

Abstract (english)

The aim of this work was to examine the influence of different air flows during the cultivation of the mold Mucor indicus DSM 2185 on corn cob hydrolysates in a bubbling bioreactor and to investigate the use of waste media for the cultivation of phototrophic non-sulfur purple bacteria. Mold grown in 1 L of acid hydrolyzate at an air flow of 0,5 L/min produced 5,07 g/L of ethanol and 5 g of biomass with 8,3% fatty acids whereas 11,7 g/L of ethanol and 10,8 g of biomass with 13,6% fatty acids were produced on the enzymatic hydrolyzate. More biomass and fatty acids were produced with less ethanol at higher airflows. Under anaerobic conditions 22,35 g/L of ethanol was produced. Rhodovulum adriaticum DSM 2178 and Rhodobacter capsulatus JCM 21090 were cultivated on the waste media. The synthesis of bacteriochlorophyll was monitored and an analysis of fatty acids was made.

• Keywords: Mucor indicus DSM 2185; ljubičaste nesumporne bakterije; hidrolizati lignocelulozne sirovine; biokemikalije; barbotirajući bioreaktor
• Keywords (english): Mucor indicus DSM 2185; purple non-sulfur bacteria; hydrolysates of lignocellulosic raw materials; biochemicals; bubbling bioreactor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:033925
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-30 10:16:00