Validacija metoda za određivanje teških metala (Pb, Cd, Sn) u proizvodima od voća atomskom apsorpcijskom spektrometrijom

master's thesis

• Title (english): Validation of the methods for determination of heavy metals (Pb, Cd, Sn) in fruit products by atomic absorbtion spectrometry
• Author: Hana Kučević
• Mentor: Marina Krpan (mentor)
• Committee member: Ksenija Marković (predsjednik povjerenstva)
• Committee member: Ivona Elez Garofulić (član povjerenstva)
• Committee member: Ines Panjkota-Krbavčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2023-06-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Validacija metode je postupak kojim se, na temelju rezultata ispitivanja ključnih parametara validacije, utvrđuje služi li metoda svrsi. Cilj ovog rada bio je utvrditi je li ispitivana metoda primjenjiva za određivanje koncentracije olova, kadmija i kositra u proizvodima od voća. Konkretno, ispitivani parametri su linearnost, preciznost u uvjetima ponovljivosti i intermedijarne preciznosti, točnost preko iskorištenja, granica kvantifikacije i granica detekcije. Svi parametri zadovoljavaju postavljeni kriterij osim granice detekcije (GD) za određivanje koncentracije kositra. GD za kositar, nalazi se izvan postavljenog kriterija ali to ne predstavlja problem u validaciji ove metode jer, prema zahtjevima vodiča AOAC, GD nije ključan parametar validacije. Rezultati ispitivanja u ovom radu dokazuju da ova metoda služi svrsi, odnosno da je primjenjiva za određivanje koncentracije olova, kadmija i kositra te da bi kod određivanja kositra u obzir trebalo uzeti da se vrijednosti ispod 1,25 mg/L ne mogu detektirati.

Abstract (english)

Validation is a procedure that, based on the test results of the key validation parameters, determines whether the method serves its purpose. The aim of this study was to establish if the tested method is applicable for determining the concentration of lead, cadmium and tin in fruit products. The validation parameters tested in this paper are linearity, precision under the conditions of repeatability and intermediate precision, accuracy as bias, limit of quantification and limit of detection. All parameters meet the criteria except the limit of detection (LOD) for determining the concentration of tin. The LOD of tin is beyond the required criterion, but this does not pose a problem in the validation of this method because, according to the requirements of the AOAC guide, the LOD is not the key validation parameter. The results in this paper prove that this method serves its purpose and is applicable for determining the concentration of lead, cadmium and tin, but when determining the concentration of tin, it should be considered that values below 1.25 mg/L cannot be detected.

• Keywords: teški metali; validacija; AAS; voće
• Keywords (english): heavy metals; validation; AAS; fruits
• Language: croatian
• URN:NBN: urn:nbn:hr:159:991682
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-15 11:27:11