Karakterizacija biološkog učinka vodenog ekstrakta ploda maginje (Arbutus unedo L.)

master's thesis

• Title (english): Characterization of the biological effect of aqueous strawberry tree fruit (Arbutus unedo L.) extract
• Author: Lara Butumović
• Mentor: Ksenija Durgo (mentor)
• Mentor: Ana Huđek Turković (pomoć pri izradi, neposredni voditelj)
• Committee member: Danijela Bursać Kovačević (predsjednik povjerenstva)
• Committee member: Predrag Putnik (član povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2023-03-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Obična planika (Arbutus unedo L.), u narodu poznatija kao maginja, samonikla je voćna vrsta koja se u tradicionalnoj medicini koristi u raznim ljekovitim pripravcima, budući da posjeduje antimikrobna i antioksidacijska svojstva. Sam plod biljke često je zanemaren i nedovoljno istražen, unatoč činjenici da obiluje polifenolima i vitaminima. Cilj ovog rada bio je utvrditi polifenolni sastav te istražiti biološki učinak vodenog ekstrakta maginje na stanične makromolekule, odabrane tumorske stanične linije epitela probavnog sustava te na patogene bakterije (Escherichia coli, Staphyloccocus aureus) i probiotičku bakteriju (Lactobacillus plantarum). Dobiveni rezultati pokazali su kako citotoksičnost ekstrakta ovisi o vrsti stanica, kao i o vremenu izloženosti. Najveći prooksidacijski učinak uočen je kod koncentracija sukladnim dnevnoj preporuci. Utvrđen je i protektivni učinak na genetički materijal prilikom oksidacijskog oštećenja uzrokovanog UV zračenjem, te snažan antimikrobni učinak na patogene bakterije.

Abstract (english)

Arbutus unedo L., popularly known as strawberry tree fruit, is a wild fruit species used in folk medicine for various therapeutic compounds, as it is known for its antimicrobial and antioxidant properties. The fruit of the plant itself is often neglected and insufficiently studied, although it is rich in polyphenols and vitamins. The aim of this work was to determine the polyphenolic composition of the strawberry tree fruit, as well as to investigate the biological effect of the aqueous strawberry tree fruit extract on cellular macromolecules, selected tumor cell lines of the digestive system epithelium, and on pathogenic bacteria (Escherichia coli, Staphyloccocus aureus) and probiotics (Lactobacillus plantarum). The obtained results showed that the cytotoxicity of the extract depends on the type of cells as well as on the duration of their exposure to the extract. The highest prooxidant effect was observed at concentrations corresponding to the daily recommendation. A protective effect on genetic material in oxidative damage caused by UV radiation and a strong antimicrobial effect on pathogenic bacteria were also determined.

• Keywords: maginja; obična planika; antioksidacijsko djelovanje; citotoksičnost; genotoksičnost
• Keywords (english): strawberry tree; Arbutus unedo L.; antioxidant activity; cytotoxicity; genotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:396714
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-21 08:47:17