Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita

master's thesis

• Title (english): Analysis of lysozyme stability in eutectic solvents based on osmolytes
• Author: Petra Lacić
• Mentor: Marina Cvjetko Bubalo (mentor)
• Committee member: Ivana Radojčić Redovniković (predsjednik povjerenstva)
• Committee member: Ana Jurinjak Tušek (član povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-11-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Proučavanjem živih sustava identificiran je specifičan mehanizam zaštite organizma u stresnim uvjetima koji se oslanja na male neutralne i netoksične molekule poznate pod imenom osmoliti. Osmoliti, osim što djeluju kao obrambeni mehanizam, imaju ključnu ulogu u održavanju termodinamičke stabilnosti biomakromolekula bez narušavanja njihove funkcionalnosti. S obzirom da se smatraju „kompleksnim adaptivnim sustavima” koje karakteriziraju emergentna svojstva, nedavno je otkrivena povezanost sa niskotemperaturnim eutektičkim otapalima koja učinkovito repliciraju prirodno okruženje različitih biomolekula, a ujedno imaju slična svojstva osmolita. U ovom radu je ispitan utjecaj niskotemperaturnih eutektičkih otapala na bazi osmolita (osmoDES) na toplinski induciranu agregaciju i termodinamičku stabilnost proteina lizozima pri ekstremnim temperaturama. S obzirom na rezultate, pripravljeni osmoDES-ovi imaju značajan potencijal za primjenu kao tekući skladišni medij proteina zbog svojih izuzetnih stabilizacijskih sposobnosti.

Abstract (english)

By studying living systems, a specific mechanism for protecting the organism in stressful conditions has been identified, which relies on small neutral and non-toxic molecules known as osmolytes. Osmolytes, apart from acting as a defense mechanism, play a crucial role in maintaining the thermodynamic stability of biomacromolecules without compromising their functionality. Considering them as „complex adaptive systems” characterized by emergent properties, a recent connection with eutectic solvents based on osmolytes (osmoDES) has been discovered, effectively replicating the natural environment of various biomolecules while sharing similar osmolyte properties. In this study, the impact of osmoDES on thermally induced aggregation and thermodynamic stability of lysozyme protein at extreme temperatures was investigated. Based on the results, the prepared osmoDES exhibit significant potential for application as a liquid storage medium for proteins due to their exceptional stabilizing capabilities.

• Keywords: osmoliti; niskotemperaturna eutektička otapala; stabilizacija proteina; agregacija
• Keywords (english): osmolytes; low temperature deep eutectic solvents; protein stabilisation; aggregation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:189608
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-23 10:35:53