Određivanje udjela slobodnih aminokiselina u listu raštike LC-MS/MS metodom

master's thesis

• Title (english): Determination of free amino acid content in kale leaf by LC-MS/MS method
• Author: Diana Grizelj
• Mentor: Mirjana Hruškar (mentor)
• Mentor: Nikola Major (komentor)
• Mentor: Tvrtko Karlo Kovačević (pomoć pri izradi, neposredni voditelj)
• Committee member: Marina Krpan (predsjednik povjerenstva)
• Committee member: Nikola Major (član povjerenstva)
• Committee member: Verica Dragović-Uzelac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2023-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Aminokiseline su primarni biljni metaboliti, ključne su za organizam same po sebi, sudjeluju u izgradnji stanica te brojim fiziološkim procesima. Mogu se nalaziti u slobodnom obliku ili vezane. Cilj ovoga rada bio je odrediti udjele slobodnih aminokiselina u listu raštike (Brassica oleracea L. var. acephala) tekućinskom kromatografijom visoke djelotvornosti u sprezi sa spektrometrijom masa (LC-MS/MS). Identificirano je i kvantificirano ukupno 17 slobodnih aminokiselina; alanin, arginin, asparaginska kiselina, cistein, glutaminska kiselina, glicin, histidin, izoleucin, leucin, lizin, metionin, fenilalanin, prolin, serin, treonin, tirozin i valin. Prolin je najzastupljenija slobodna aminokiselina u listu raštike s prosječnom koncentracijom koja se kretala od 2104 mg/kg suhe tvari do 3237 mg/kg suhe tvari. Cistein je aminokiselina s najnižom prosječnom koncentracijom od 0,116 mg/kg suhe tvari do 0,338 mg/kg suhe tvari.

Abstract (english)

Amino acids are the primary herbal metabolites, which are essential to the organism itself, participate in cell construction and many physiological processes. Amino acids can be in free or bound form. The aim of this study was to determine the free amino acid content in the kale leaf (Brassica oleracea L. var. acephala) by high performance liquid chromatography coupled with mass spectrometry (LC-MS/MS). A total of 17 free amino acids have been identified and quantified; alanine, arginine, asparaginic acid, cysteine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serin, threonine, tyrosine and valine. Proline is the most abundant free amino acid in the kale leaf with an average concentration in range from 2104 mg/kg dry matter to 3237 mg/kg dry matter. Cysteine is an amino acid with a lowest average concentration in range from 0.116 mg/kg dry matter to 0.338 mg/kg dry matter.

• Keywords: ESI-LC-MS/MS; ciljana analiza; slobodne aminokiseline; Brassica oleracea L. var. acephala
• Keywords (english): ESI-LC-MS/MS; targeted analysis; free amino acids; Brassica oleracea L. var. acephala
• Language: croatian
• URN:NBN: urn:nbn:hr:159:626061
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-28 12:58:51