Istraživanje utjecaja suplementacije prebiotika na sastav mikrobioma sportaša

master's thesis

• Title (english): Research of the influence of prebiotic supplementation on the microbial composition of athletes
• Author: Ira Renko
• Mentor: Antonio Starčević (mentor)
• Mentor: Jurica Žučko (pomoć pri izradi, neposredni voditelj)
• Committee member: Anita Slavica (predsjednik povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Committee member: Jurica Žučko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2023-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Crijevna mikrobiota dinamičan je sustav, podložan promjenama pod utjecajem vanjskih i unutarnjih čimbenika. Ima važnu ulogu u zdravlju domaćina jer je putem krvi povezana sa svim dijelovima organizma. U ovom radu analiziran je utjecaj konzumacije prebiotika u periodu od četiri tjedna na sastav mikrobioma košarkaša. Genetički materijal izoliran je iz uzoraka fecesa 18 ispitanika pri čemu je polovica ispitanika predstavljala kontrolnu skupinu. Rezultati sekvenciranja obrađeni su QIIME2 računalnim alatom i prikazani na taksonomskim razinama domena, koljeno, razred, red, porodica, rod i vrsta. ANCOM analiza pokazala je kako na taksonomskim razinama roda i vrste postoji statistički značajna razlika između sportaša koji su konzumirali prebiotike i koji ih nisu konzumirali. Izračunom alfa i beta raznolikosti utvrđeno je kako ne postoji statistički značajna razlika između sportaša koji su konzumirali prebiotike i koji ih nisu konzumirali. Međutim, ustanovljena je statistički značajna razlika među svim ispitanicima, bez obzira na konzumaciju prebiotika.

Abstract (english)

Intestinal microbiota is a dynamic system, subjected to changes under the influence of external and internal factors. It plays an important role in the host's health because it is connected to all parts of the body through the blood. This thesis analyses the influence of the consumption of prebiotics in a period of four weeks on the microbial composition of basketball players. Genetic material was isolated from the feces samples of 18 subjects, half of which represented the control group. Sequencing results were processed with the QIIME2 computer tool and displayed at the taxonomic levels of domain, genus, class, order, family, genus and species. ANCOM analysis showed that at the taxonomic levels of genus and species, there is a statistically significant difference between athletes who consumed prebiotics, and those who did not. By calculating the alpha and beta diversity, no statistically significant difference between athletes who consumed prebiotics and those who did not was found. However, there is a statistically significant difference among all respondents regardless of the prebiotics consumption.

• Keywords: analiza crijevne mikrobiote; mikrobiota; bioinformatička analiza; prebiotici; taksonomija.
• Keywords (english): intestinal microbiota analysis; microbiota; bioinformatics analysis; prebiotics; taxonomy
• Language: croatian
• URN:NBN: urn:nbn:hr:159:648853
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-04 13:23:12