Određivanje citotoksičnog, proliferacijskog, prooksidacijskog i genotoksičnog učinka mješavine odabranih pesticida na humanim kontinuiranim staničnim linijama karcinoma želuca i jetre

master's thesis

• Title (english): Determining cytotoxic, proliferative, prooxidative and genotoxic effect of pesticide mixture on human continuous gastric and liver carcinoma cell lines
• Author: Katarina Ivona Odak
• Mentor: Ksenija Durgo (mentor)
• Committee member: Kristina Radošević (predsjednik povjerenstva)
• Committee member: Ivana Kmetič (član povjerenstva)
• Committee member: Marina Cvjetko Bubalo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2023-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Pesticidi su kemijske tvari u širokoj suvremenoj upotrebi zbog čije se sve učestalije primjene povećava izloženost opće populacije. Iz tog razloga, provodi se sve veći broj studija, in vitro i in vivo koje ispituju njihove potencijalne toksične učinke. U ovom su istraživanju korištene dvije humane stanične linije karcinoma želuca i jetre – AGS i Hep G2. Tretirane su mješavinom koja se sastojala od osam pesticida, među kojima su 4 herbicida (glifosat, S-metolaklor, nikosulfuron i terbutilazin), 3 insekticida (acetamiprid, deltametrin i spinosad) i 1 fungicid (kaptan). Smjesa pesticida uzrokovala je citotoksični i prooksidacijski učinak kod obje stanične linije u ovisnosti o koncentraciji, a snažnije djelovanje uočeno je u Hep G2 stanicama koje su metabolički aktivne. Povećanjem koncentracije mješavine pesticida došlo je do smanjenja stanične diobe kod obje stanične linije. Pri nižim su koncentracijama AGS stanice pokazivale bolju proliferacijsku sposobnost, dok porastom koncentracije dolazi do selekcije rezistentnih klonova Hep G2 stanica. Mješavina pesticida uzrokovala je povećanje postotka DNA repa kometa u staničnoj liniji Hep G2, dok se za AGS staničnu liniju nije pokazala genotoksičnom. Potrebna su dodatna istraživanja kako bi se objasnio učinak pesticida na ovim staničnim linijama te rastumačio njihov mehanizam obrane i prilagodbe ovakvim ksenobioticima.

Abstract (english)

Pesticides are chemical substances widely used in modern times, and their increasing use exposes the general population to potential risks. For this reason, an increasing number of studies, both in vitro and in vivo, are being conducted to examine their potential toxic effects. In this research, two human cell lines of gastric and liver cancer – AGS and Hep G2 – were used. They were treated with a mixture consisting of eight pesticides, including 4 herbicides (glyphosate, S-metolachlor, nicosulfuron and terbuthylazine), 3 insecticides (acetamiprid, deltamethrin, and spinosad), and 1 fungicide (captan). The pesticide mixture caused cytotoxic and prooxidative effects on both cell lines, depending on the concentration, with stronger effects observed in Hep G2 cells, which are metabolically active. Increasing the concentration of the pesticide mixture led to a decrease in cell division in both cell lines. At lower concentrations, AGS cells showed better proliferation ability, while higher concentrations led to the selection of resistant clones in Hep G2 cells. The pesticide mixture caused an increase in the percentage of DNA comet tails in the Hep G2 cell line, while it did not show genotoxic effects on the AGS cell line. Additional research is needed to explain the effects of pesticides on these cell lines and to understand their defense mechanisms and adaptation to such xenobiotics.

• Keywords: mješavina pesticida; AGS stanična linija; Hep G2 stanična linija; citotoksičnost; prooksidacijsko djelovanje; genotoksičnos
• Keywords (english): pesticide mixture; AGS cell line; Hep G2 cell line; cytotoxicity; prooxidative effect; genotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:534228
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-06 11:53:34