Antistafilokokni učinak Lactiplantibacillus plantarum sojeva iz mikrobiote majčinog mlijeka

master's thesis

• Title (english): Antistaphylococcal effect of Lactiplantibacillus plantarum strains from human milk microbiota
• Author: Helena Rak
• Mentor: Jasna Novak (mentor)
• Mentor: Katarina Butorac (pomoć pri izradi, neposredni voditelj)
• Mentor: Nina Čuljak (pomoć pri izradi, neposredni voditelj)
• Committee member: Andreja Leboš-Pavunc (predsjednik povjerenstva)
• Committee member: Ana Bielen (član povjerenstva)
• Committee member: Ksenija Markov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2023-07-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

S aspekta mikrobne ekologije, majčino mlijeko se može promatrati kao ekosustav, jer u svom sadržaju, osim brojnih bioaktivnih molekula sadrži i specifičnu mikrobnu zajednicu koja je potencijalni izvor probiotičkih sojeva. Staphylococcus epidermidisje dio mikrobiote kože zdrave osobe, no prisutnost u mikrobioti majčinog mlijeka se povezuje s laktacijskim mastitisom. Kod Staphylococcus spp. koji uzrokuju mastitis, nerijetko je prisutna višestruka rezistencija na antibiotike, što uz mogućnost ovih bakterija da formiraju biofilm na biotičkim površinama, ograničava učinkovitosti antibiotičke terapije. Stoga je cilj ovog rada bio ustanoviti alternativne pristupe primjene probiotičkih bakterija kao strategije za profilaksu i/ili terapiju laktacijskog mastitisa. Istraživanja su provedena s potencijalnim probiotičkim sojevima Lactiplantibacillus plantarum iz mikrobiote majčinog mlijeka. Preliminarno je okarakterizirana pojavnost antibiotičke rezistencije kod populacije Staphylococcus sojeva te je utvrđena niska učestalost fenotipske rezistencije. Analizirani sojevi L. plantarum MB18, KR19 i MC19 iskazuju potencijal inhibicije rasta, ali i inhibicijski učinak na formiranje biofilma S. epidermidis. Također, ustanovljeno je da odabrani L. plantarum sojevi iz mikrobiote majčinog mlijeka su potencijalni producenti plantaricina koji uz sintetiziranu mliječnu kiselinu, posreduju u antistafilokoknom učinku.

Abstract (english)

In the context of microbial ecology, human milk is viewed as an ecosystem, because in its content, in addition to numerous bioactive molecules, it also contains specific microbes that are a potential source of probiotic strains. Staphylococcus epidermidis is a natural inhabitant of the skin microbiota of a healthy person. However, its presence in the microbiota of human milk is frequently associated with lactational mastitis. Staphylococcus spp., the causative agent of mastitis, exert multiple resistance to antibiotics is often present, which, along with the capacity to form a bacterial biofilm, limits the effectiveness of antibiotic therapy. Therefore, the aim of this thesis was to assess alternative approaches to the use of probiotic bacteria as a strategy for the prophylaxis or therapy of lactational mastitis. Research was conducted with potential probiotic strains of Lactiplantibacillus plantarum from human milk microbiota. The incidence of antibiotic resistance in the Staphylococcus population was preliminarily characterized and a low frequency of phenotypic resistance was determined. Among analyzed strains, L. plantarum MB18, KR19 and MC19 inhibited both the growth and biofilm formation of S. epidermidis. Also, it was established that selected L. plantarum strains from the human milk microbiota are potential plantaricins producers, which mediate antistaphylococcal effect in addition to the synthesized lactic acid.

• Keywords: Lactiplantibacillus plantarum; Staphylococcus epidermidis; mikrobiota majčinog mlijeka; biofilm; plantaricini
• Keywords (english): Lactiplantibacillus plantarum; Staphylococcus epidermidis; human milk microbiota; biofilm; plantaricins
• Language: croatian
• URN:NBN: urn:nbn:hr:159:193478
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-11 08:33:18