Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51

master's thesis

• Title (english): Identification and quantification of bioactive peptides produced by Lactococcus lactis ZGBP5-51
• Author: Anamarija Rušanac
• Mentor: Blaženka Kos (mentor)
• Mentor: Ana Butorac (komentor)
• Mentor: Katarina Butorac (pomoć pri izradi, neposredni voditelj)
• Committee member: Andreja Leboš-Pavunc (predsjednik povjerenstva)
• Committee member: Ana Butorac (član povjerenstva)
• Committee member: Anita Slavica (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog diplomskog rada bio je identificirati i kvantificirati bioaktivne peptide nastale u uzorcima mlijeka tijekom fermentacije proteolitičkom aktivnošću radnog soja Lactococcus lactis ZGBP5-51. Identifikacija bioaktivnih peptida provedena je spregnutom tehnikom tekućinske kromatografije i spektrometrije masa LC-MALDI-TOF/TOF, a relativna kvantifikacija identificiranih bioaktivnih peptida spregnutom tehnikom tekućinske kromatografije, višestrukog praćenja reakcije i spektrometrije masa LC-MRM-MS. Ne-ciljanom analizom identificirani su bioaktivni peptidi TKVIPYVRYL, VYPFPGPIPN, ARHPHPHLSFM, KYIPIQYVL, QEPVLGPVRGPFPIIV, IQPKTKVIPYVRYL i YQEPVLGPVRGPFPIIV, dok su ciljanom analizom identificirana još tri bioaktivna peptida: RDMPIQAF, SQSKVLPVPQKAVPYPQ i LYQEPVLGPVRGPFPIIV. U znanstvenoj literaturi za navedene peptide utvrđeno je i opisano ACE-inhibitorno (antihipertenzivno), antitrombotično, antioksidativno, antimikrobno i imunomodulatorno djelovanje. Ciljanom analizom uspješno je kvantificirano osam prethodno identificiranih bioaktivnih peptida. Konačno, statistički značajan porast koncentracije, nakon 48 sati fermentacije, dokazan je za šest peptida: RDMPIQAF, VYPFPGPIPN, QEPVLGPVRGPFPIIV, SQSKVLPVPQKAVPYPQ, YQEPVLGPVRGPFPIIV i ARHPHPHLSFM.

Abstract (english)

The aim of this thesis was to identify and quantify bioactive peptides produced during milk fermentation by proteolitic activity of the strain Lactococcus lactis ZGBP5-51. Identification of bioactive peptides was performed using the liquid chromatography and mass spectrometry LC-MALDI TOF/TOF technique, while relative quantification of identified bioactive peptides was performed using the liquid chromatography, multiple reaction monitoring and mass spectrometry LC-MRM-MS technique. The non-targeted analysis identified bioactive peptides TKVIPYVRYL, VYPFPGPIPN, ARHPHPHLSFM, KYIPIQYVL, QEPVLGPVRGPFPIIV, IQPKTKVIPYVRYL, and YQEPVLGPVRGPFPIIV, while the targeted analysis identified three additional bioactive peptides: RDMPIQAF, SQSKVLPVPQKAVPYPQ, and LYQEPVLGPVRGPFPIIV. ACE-inhibitory (antihypertensive), antithrombotic, antioxidant, antimicrobial, and immunomodulatory effects have been determined and described in the scientific literature for the aforementioned peptides. Through targeted analyses, eight previously identified bioactive peptides were successfully quantified. Finally, statistically significant increase in concentration, after 48 hours of fermentation, was detected for six previously identified bioactive peptides: RDMPIQAF, VYPFPGPIPN, QEPVLGPVRGPFPIIV, SQSKVLPVPQKAVPYPQ, YQEPVLGPVRGPFPIIV and ARHPHPHLSFM.

• Keywords: bioaktivni peptidi; Lactococcus lactis ZGBP5-51; proteolitički sustav; kazein
• Keywords (english): bioactive peptides; Lactococcus lactis ZGBP5-51; proteolytic system; casein
• Language: croatian
• URN:NBN: urn:nbn:hr:159:495075
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-11 08:51:26