Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote

master's thesis

• Title (english): Analysis of sequencing results and association between stool consistency and frequency and gut microbiota composition
• Author: Ivana Eranović
• Mentor: Jurica Žučko (mentor)
• Committee member: Ivana Rumora Samarin (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2023-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Crijevna mikrobiota vrlo je dinamičan sustav, a na njegov sastav značajno utječu različiti obrasci prehrane, ali i mnogi drugi čimbenici. Zanemarivanje utjecaja pojedinih čimbenika na sastav mikrobiote može značajno utjecati na interpretaciju rezultata dobivenih istraživanjima. Učestalost i konzistencija stolice su mjere intestinalnog tranzitnog vremena – čimbenika koji determinira sadržaj vode i nutrijenata u crijevu te time oblikuje uvjete mikrobnog staništa. Cilj ovoga rada bio je povezati učestalost i konzistenciju stolice sa sastavom crijevne mikrobiote. Istraživanje je provedeno na 59 ispitanika od kojih su prikupljeni uzorci stolice iz kojih je određivan sastav mikrobiote sekvenciranjem 16S rRNA gena na Illumina MiSeq uređaju, a informacije o učestalosti i konzistenciji stolice dobiveni su ispunjavanjem upitnika. Za analizu podataka dobivenih sekvenciranjem korišten je program QIIME2 te DADA2 metoda pročišćavanja podataka. Prema rezultatima ove analize nije uočena značajna povezanost sastava crijevne mikrobiote s učestalosti i/ili konzistencijom stolice na promatranoj skupini ispitanika.

Abstract (english)

Gut microbiota is a very dynamic system, and its composition is strongly affected by individual’s diet pattern, as well as numerous other factors. Disregarding the impact of these factors on microbiota composition can significantly affect the interpretation of results obtained in the study. Stool consistency and frequency are used as proxies for intestinal transit time – a factor determining water and nutrient content in the intestine and, consequently, shaping the conditions of the microbial habitat. The aim of this study was to observe connection between stool consistency and frequency and gut microbiota composition. Study was conducted on 59 subjects from whom stool samples were collected and analyzed by sequencing 16S rRNA gene on Illumina MiSeq platform to determine microbiota composition. Data considering stool consistency and frequency was collected via online questionnaire. QIIME2 pipeline was used for analysis of sequencing data including DADA2 method for denoising step. The results of this study showed no significant association between stool consistency and/or frequency and gut microbiota composition in the observed group of subjects.

• Keywords: mikrobiota; stolica; QIIME2; varijante sekvence amplikona (ASV)
• Keywords (english): microbiota; stool; QIIME2; amplicon sequence variants (ASV)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:137052
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-14 11:29:24