Biodostupnost i antioksidacijska aktivnost polifenolnih spojeva u 3D ispisanim funkcionalnim proizvodima na bazi jagode tijekom in vitro probave

master's thesis

• Title (english): Bioavailability and antioxidant activity of polyphenol compounds in 3D printed functional products based on strawberry during in vitro digestion
• Author: Paula Kralj-Vrsalović
• Mentor: Danijela Bursać Kovačević (mentor)
• Mentor: Ksenija Durgo (pomoć pri izradi, neposredni voditelj)
• Mentor: Ana Huđek Turković (pomoć pri izradi, neposredni voditelj)
• Mentor: Anica Bebek Markovinović (pomoć pri izradi, neposredni voditelj)
• Committee member: Ksenija Durgo (predsjednik povjerenstva)
• Committee member: Maja Benković (član povjerenstva)
• Committee member: Ana Jurinjak Tušek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2023-12-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Povećanje svijesti potrošača o unaprjeđenju zdravlja rezultiralo je potrebom za razvojem inovativnih funkcionalnih prehrambenih proizvoda. Tehnologija 3D ispisa nudi mogućnost razvoja širokog spektra takvih proizvoda okrenutih ciljanim potrebama potrošača. U ovom istraživanju ispitana je biodostupnost i antioksidacijska aktivnost polifenolnih spojeva u 3D ispisanim proizvodima na bazi jagode tijekom in vitro probave koja se sastojala od oralne, želučane i intestinalne faze. Analiziranu su uzorci kaše jagode i 3D ispisani uzorci s pšeničnim i s kukuruznim škrobom. Sadržaj polifenola je određen Folin Ciocalteu metodom, dok je antioksidacijski kapacitet određen FRAP i DPPH metodama. Po provedenoj in vitro probavi, u uzorcima kaše jagode uočen je porast antioksidacijskog kapaciteta, dok isti učinak nije bio prisutan u 3D uzorcima. U svim uzorcima udio polifenola pozitivno utječe na antioksidacijski kapacitet. S obzirom na sadržaj polifenola i antioksidacijski kapacitet 3D ispisani proizvodi na bazi jagode ukazuju na širi potencijal 3D ispisa u razvoju inovativnih proizvoda u kategoriji funkcionalne hrane.

Abstract (english)

Increasing consumer awareness of improving health has resulted in the need to develop innovative functional food products. 3D printing technology (3DP) offers the opportunity to develop a wide range of such products to meet the targeted needs of consumers. In this study, the bioavailability and antioxidant activity of polyphenolics in 3DP strawberry-based products were tested during in vitro digestion, which consisted of oral, a gastric and intestinal phase. Samples of strawberry pulp and 3DP samples with wheat and corn starch were analyzed. The polyphenol content was determined using Folin Ciocalteu method and antioxidant capacity was determined by FRAP and DPPH assays. After in vitro digestion, an increase in antioxidant capacity was observed in pulp samples, while same effect was not present in 3DP samples. Polyphenolic content had positive effect on antioxidant capacity. Considering the polyphenolic content and antioxidant capacity, 3DP strawberry-based products show great potential for the development of innovative functional foods.

• Keywords: 3D ispis; in vitro probava; jagoda (Fragaria ananassa x Duch); bioaktivni spojevi; antioksidacijski kapacitet
• Keywords (english): 3D printing; in vitro digestion; strawberry (Fragaria ananassa x Duch); bioactive compounds; antioxidant capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:786680
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-18 08:50:40