Identifikacija bioaktivnih spojeva i peptida dobivenih iz mikroalge Arthrospira platensis primjenom proteomskog i metabolomskog pristupa

master's thesis

• Title (english): Identification of bioactive compounds and peptides obtained from microalgae Arthrospira platensis by application of proteomic and metabolomic approach
• Author: Dora Švegović
• Mentor: Anita Horvatić (mentor)
• Mentor: Josipa Kuleš (komentor)
• Committee member: Antonio Starčević (predsjednik povjerenstva)
• Committee member: Josipa Kuleš (član povjerenstva)
• Committee member: Mojca Čakić Semenčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Physical Chemistry and Corrosion); Zagreb
• Defense date and country: 2023-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mikroalga Arthrospira platensis (komercijalnog naziva spirulina) sve se češće koristi kao dodatak prehrani, nutraceutik, sastojak kozmetičkih preparata i lijekova te sirovina za proizvodnju biogoriva. Cilj ovog rada bio je proteomskim i metabolomskim pristupom temeljenim na spektrometriji masa u kombinaciji s najsuvremenijim bioinformatičkim alatima identificirati bioaktivne spojeve i peptide koji se mogu dobiti iz mikroalge Arthrospira platensis, kao i alergene, budući da su alergije na hranu u sve većem porastu. Koristeći rezultate proteomske analize, pomoću servera PeptideRanker predviđene su potencijalne bioaktivnosti identificiranih peptida, dok je pomoću baze Allergome identificiran alergen C-fikocijanin. Rezultati metabolomske analize korišteni su za pretraživanje baze COCONUT, pri čemu je identificirano 68 bioaktivnih metabolita. Na osnovu dobivenih rezultata zaključeno je da, zbog bioaktivnih molekula koje sadrži, Arthrospira platensis ima pozitivno djelovanje na ljudsko zdravlje pri čemu se ističu antioksidativno, protuupalno i antibakterijsko djelovanje. S druge strane, ova mikroalga može predstavljati prijetnju za zdravlje kod preosjetljivih pojedinaca.

Abstract (english)

The microalga Arthrospira platensis (commonly called spirulina) is increasingly used as a food supplement, nutraceutical, ingredient in cosmetics and medicines, and raw material in production of biofuel. The aim of this thesis was to identify bioactive compounds and peptides that can be extracted from Arthrospira platensis, using mass spectrometry-based proteomics, metabolomics and sophisticated bioinformatic tools, as well as allergens, since the constant growth of allergies frequency could be observed. Using the results of proteomic analysis and the PeptideRanker server, the potentially bioactive peptides were predicted. Furthermore, C-phycocyanin was identified as allergen by Allergome database search. Metabolomics results were used for COCONUT database search, enabling the identification of 68 bioactive metabolites. Finally, it can be concluded that, because of the bioactive molecules it contains, the microalga Arthrospira platensis has a positive impact on human health, showing antioxidant, antitumor and antibacterial activities. However, it can pose a potential threat for sensitive individuals.

• Keywords: spirulina; bioaktivni peptidi; proteomika; metabolomika; spektrometrija masa
• Keywords (english): spirulina; bioactive peptides; proteomics; metabolomics; mass spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:687221
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-27 12:13:49