Utjecaj ekstrakcijskog otapala na antioksidacijsku aktivnost nusproizvoda egzotičnog voća

undergraduate thesis

• Title (english): Effect of extraction solvent on antioxidant activity of exotic fruit byproducts
• Author: Otilija Štargl
• Mentor: Ivona Elez Garofulić (mentor)
• Mentor: Erika Dobroslavić (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2024-09-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nusproizvodi egzotičnog voća predstavljaju prehrambeni otpad te se koriste kao sirovina za proizvodnju novih ili obogaćivanje postojećih proizvoda u prehrambenoj, farmaceutskoj i industriji do dataka prehrani. Nusproizvodi avokada i banane sadrže visoke razine raznih bioaktivnih tvari koje imaju pogodan utjecaj na zdravlje, industriju i ekologiju. Cilj ovog rada bio je ispitati utjecaj ekstrakcijskog otapala na antioksidacijsku aktivnost kore avokada i banane. U svrhu ispitivanja utjecaja ekstrakcijskog otapala, primijenjena su tri različita duboka eutektička otapala s 10 i 30 % vode te je uspoređena njihova učinkovitost s 30 %-tnom vodenom otopinom etanola kao primjerom konvencionalnog otapala. Za od ređivanje antioksidacijske aktivnosti korištene su tri spektrofotometrijske metode, DPPH, FRAP i ABTS. Neovisno o metodi, kao najučinkovitija ekstrakcijska otapala su se pokazala kolin klorid i glicerol (1:3) s 30 % H₂O te kolin klorid i mliječna kiselina (1:3) s 30 % H₂O za ekstrakte avokada, a za ekstrakte banane ista ekstrakcijska otapala, ali s 10 % H₂O. Prilikom ekstrakcije kore avokada, duboka eutektička otapala su bila podjednako učinkovita kao 30 %-tna vodena otopina etanola, dok je kod kore banane etanolni ekstrakt imao značajno nižu antioksidacijsku aktivnost. Može se zaključiti da duboka eutekti čka otapala imaju visok potencijal primjene u izolaciji komponenata s antioksidacijskom aktivnošću iz kore avokada i banane.

Abstract (english)

Exotic fruit by-products represent food waste and are used as raw material for the produc tion of new or enrichment of existing products in the food, pharmaceutical and food supplement indus tries. Avocado and banana by-products contain a high level of various bioactive substances that have a beneficial effect on health, industry and ecology. The aim of this work was to examine the influence of the extraction solvent on the antioxidant activity of avocado and banana peels. In order to evaluate the influence of the extraction solvent, three different deep eutectic solvents with 10 and 30% water were used and their effectiveness was compared with a 30% aqueous ethanol solution as an example of a conventional solvent. Three spectrophotometric methods, DPPH, FRAP and ABTS were used to determine antioxidant activity. Regardless of the method, the most effective extraction solvents were choline chloride and glycerol (1:3) with 30% H₂O and choline chloride and lactic acid (1:3) with 30% H₂O for avocado extracts, and the same for banana extracts. extraction solvents, but with 10% H₂O. When extracting avocado peel, deep eutectic solvents were equally effective as 30% aqueous ethanol solution, while with banana peel ethanol extract had significantly lower antioxidant activity. It can be concluded that deep eutectic solvents have a high application potential in the isolation of components with antioxidant activity from avocado and banana peels.

• Keywords: avokado; banana; antioksidacijska aktivnost; ekstrakcija; duboka eutektička otapala
• Keywords (english): avocado; banana; antioxidant activity; extraction; deep eutectic solvents
• Language: croatian
• URN:NBN: urn:nbn:hr:159:335942
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-23 09:49:11