Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke

master's thesis

• Title (english): The effect of curcumin and pyrazole analogue of curcumin under conditions of nutrient deprivation on the viability and proliferation of breast cancer cells
• Author: Laura Režek
• Mentor: Ivana Kmetič (mentor)
• Mentor: Marina Miletić (pomoć pri izradi, neposredni voditelj)
• Mentor: Teuta Murati (pomoć pri izradi, neposredni voditelj)
• Committee member: Teuta Murati (predsjednik povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2024-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj rada bio je ispitati učinak nutritivne deprivacije, kao i učinak kurkumina (CRC) i pirazolnog analoga kurkumina (PC) (2,5 – 100 µM) u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju MCF 7 stanične linije, te na indukciju stanične smrti autofagijom i apoptozom. Stanična vijabilnost praćena je metodama MTT, Neutral Red i Trypan Blue, dok je analiza stanične smrti provedena metodom protočne citometrije. Uzgoj stanica u DMEM High Glucose i DMEM Low Glucose hranjivim medijima razrijeđenim PBS ili HBSS puferom uzrokovao je značajno smanjenje proliferacije stanica određeno MTT metodom, pri čemu koncentracija dostupne glukoze nije bila ograničavajući parametar. Nutritivna deprivacija potaknula je autofagiju kod samo jednog uzorka, dok je apoptoza bila blago inducirana kod svih i to nešto jače kod primjene medija s nižom koncentracijom glukoze. CRC i PC uzrokovali su smanjenje stanične vijabilnosti u ovisnosti u dozi, pri čemu su se parametri poput načina i vremena tretiranja stanica (stanice nacijepljene u mediju za uzgoj u koji je dodan CRC ili PC; stanice tretirane s CRC-om ili PC-om nakon 24h (odnosno nakon formiranog monosloja)) pokazali važnim za konačne citotoksične učinke navedenih spojeva. Nutritivna deprivacija pojačala je citotoksično djelovanje CRC-a, što je potvrđeno metodama MTT, Neutral Red i Trypan Blue. Udio stanica koje odumiru autofagijom ili apoptozom značajno je porastao povećanjem koncentracije CRC-a (5 – 100 µM) u uvjetima nutritivne deprivacije.

Abstract (english)

The aim of this study was to examine the effect of nutrient deprivation, as well the effect of curcumin (CRC) and pyrazole analogue of curcumin (PC) (2,5 – 100 µM) under conditions of nutrient deprivation on the viability and proliferation of the MCF-7 cells, and on the induction of cell death by autophagy and apoptosis. Cell viability was determined using MTT, Neutral Red and Trypan Blue methods, while cell death analysis was performed using the flow cytometry method. DMEM High Glucose and DMEM Low Glucose media diluted with PBS or HBSS caused a significant decrease in cell proliferation, which was determined by MTT method, while the concentration of available glucose was not the limiting parameter. Nutrient deprivation stimulated autophagy in only one sample, while apoptosis was mildly induced in all of them and somewhat stronger when using media with a lower glucose concentration. CRC and PC caused a decrease in cell viability in a dose-dependent manner, with parameters such as conditions and time of cell treatment (cells inoculated in culture medium to which CRC or PC was added; cells treated with CRC or PC after 24h (that is, after monolayer formation)) proved to be important for the final cytotoxic effects of the mentioned compounds. Nutrition deprivation enhanced the cytotoxic effect of CRC, which was confirmed by the MTT, Neutral Red and Trypan Blue methods. The proportion of cells that undergo cell death by autophagy or apoptosis increased significantly in conditions of nutrient deprivation as concentration of CRC (5 – 100 µM) increased.

• Keywords: nutritivna deprivacija; kurkumin; pirazolni analog kurkumina; autofagija; apoptoza
• Keywords (english): nutrient deprivation; curcumin; pyrazole analogue of curcumin; autophagy; apoptosis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:020997
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-21 12:48:06