Primjena blisko infracrvene spektroskopije u detekciji fungicida Chromosul i Cuprablau prisutnih u jabukama

master's thesis

• Title (english): Application of near infrared spectroscopy in the detection of Chromosul and Cuprablau fungicides present in apples
• Author: Mihaela Severec
• Mentor: Tamara Jurina (mentor)
• Committee member: Jasenka Gajdoš Kljusurić (predsjednik povjerenstva)
• Committee member: Maja Benković (član povjerenstva)
• Committee member: Danijela Bursać Kovačević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Laboratory for Measurement, Regulation and Automatisation); Zagreb
• Defense date and country: 2024-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

U ovom radu glavni cilj bio je istražiti učinkovitost NIR spektroskopije (engl. Near Infrared Spectroscopy, NIRs) za kvalitativno određivanje prisutnosti fungicida Chromosul 80 i Cuprablau Z 35 WG na površini jabuka. Jabuke su tretirane različitim koncentracijama istraživanih fungicida te su, tijekom trotjednog vremenskog perioda, snimane pomoću tri NIR spektrofotometara u rasponu valnih duljina λ = 904 – 2500 nm.. Primjenom NIR spektara, u kombinaciji sa analizom glavnih komponenata, uspješno su razdvojeni (i) originalni uzorci suspenzija/otopina fungicida od njihovih odgovarajućih razrjeđenja te (ii) tretirani uzorci jabuka u odnosu na netretirane (kontrolne) uzorke. Kako bi se ustanovila povezanost između NIR spektara i fizikalnih karakteristika (ukupne otopljene tvari, vodljivost, boja) suspenzija/otopina istraživanih fungicida i njihovih odgovarajućih razrjeđenja, razvijeni su PLS (engl. Partial Least Squares) regresijski modeli koji su pokazali da postoji linearna zavisnost između absorpcijskih vrijednosti u NIR području te fizikalnih karakteristika (ukupne otopljene tvari, vodljivost) istraživanih fungicida (R² ≥ 0,9).

Abstract (english)

The aim of this work was to investigate the effectiveness of Near Infrared Spectroscopy (NIRs) for the qualitative determination of the presence of fungicides Chromosul 80 and Cuprablau Z 35 WG on the surface of apples. Apples were treated with different concentrations of the fungicides and, during a three-week period, were recorded in the range λ = 904 - 2500 nm. NIR spectroscopy, in combination with Principal Component Analysis, successfully separated (i) the fungicide suspensions/solutions from their dilutions, as well as (ii) treated apple samples compared to untreated (control) samples. In order to establish the relationship between NIR spectra and physical characteristics (total dissolved solids, conductivity, color) of suspensions/solutions of the investigated fungicides and their corresponding dilutions, Partial Least Squares models were developed showing a linear relationship between NIR spectra and physical characteristics (total dissolved solids, conductivity) of the investigated fungicides (R² ≥ 0.9).

• Keywords: blisko infracrvena spektroskopija; kemometrija; fungicidi; jabuka
• Keywords (english): near-infrared spectroscopy; chemometrics; fungicides; apple
• Language: croatian
• URN:NBN: urn:nbn:hr:159:824775
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-24 08:56:55