Primjena 3D - ispisanog reaktora za provedbu tretmana pulsirajućim električnim poljem

master's thesis

• Title (english): Application of 3D printed reactor for pulsed electric field treatments
• Author: Dorian Dević
• Mentor: Tomislava Vukušić Pavičić (mentor)
• Mentor: Višnja Stulić (mentor)
• Committee member: Zoran Herceg (predsjednik povjerenstva)
• Committee member: Tonči Rezić (član povjerenstva)
• Committee member: Klara Kraljić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Food Processes Engineering); Zagreb
• Defense date and country: 2024-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

U ovom istraživanju konstruiran je protočni 3D - ispisani reaktor u kojem su provedeni predtretmani ekstrakciji bioaktivnih komponenti iz povrća. Sirovine korištene u radu su batat (Ipomoea batatas L.) i paprika (Capsicum annuum L.). Ispitivane su razlike u boji, masenim udjelima fenolnih spojeva i karotenoida te antioksidacijskih svojstava pri varijabilnim parametrima pulsirajućeg električnog polja – duljina pulsa (0,5; 1,25; 2,0 μs) te vrijeme tretmana (6; 9; 12 min), dok je jakost električnog polja bila konstantna (4,26 kV cm⁻¹). Metode korištene za analizu uzoraka su spektrofotometrijsko i HPLC određivanje udjela karotenoida, spektrofotometrijsko određivanje udjela ukupnih polifenola, antioksidacijske aktivnosti i parametara boje. U provedbi eksperimenta također je praćena promjena pH i električne provodljivosti. Najveći maseni udio β-karotena u uzorcima paprike bio je kod uzorka P2 (0,148 mg g⁻¹ s.tv.), a kod uzoraka batata u uzorku B9 (0,119 mg g⁻¹ s.tv.). Uočene su prednosti i mane konstrukcije protočnog reaktora te predložene promjene za buduće konstrukcije.

Abstract (english)

In this research, a flow-through 3D printed reactor was constructed, in which pretreatments for the extraction of bioactive components from vegetables were conducted. The raw materials used in the study were sweet potato (Ipomoea batatas L.) and pepper (Capsicum annuum L.). Differences in color, mass fractions of phenolic compounds, carotenoid compounds, and antioxidant properties were examined under variable pulsed electric field (4,26 kV cm⁻¹) parameters – pulse duration (0.5; 1.25; 2.0 μs) and treatment time (6; 9; 12 minutes). Methods used for sample analysis were spectrophotometric and HPLC determination of carotenoid content, spectrophotometric determination of total polyphenol content and antioxidant activity, as well as color parameters. During the experiment, changes in pH and electrical conductivity were also monitored. The highest β-carotene content in the pepper samples was observed in sample P2 (0.148 mg g⁻¹ d.m.), while in the sweet potato samples, it was found in sample B9 (0.119 mg g⁻¹ d.m.). The advantages and disadvantages of the flow reactor design were noted, and modifications for future designs were proposed.

• Keywords: 3D - ispis; pulsirajuće električno polje; fenolni spojevi; karotenoidi
• Keywords (english): 3D print; pulsed electric field; phenolic compounds; carotenoids
• Language: croatian
• URN:NBN: urn:nbn:hr:159:654727
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-24 09:54:56