Istraživanje utjecaja odabranih aktivnih tvari iz pesticida na ekspresiju gena primjenom in silico analiza

master's thesis

• Title (english): Research on the impact of selected active substances from pesticides on gene expression using in silico analysis
• Author: Mia Galić
• Mentor: Ksenija Durgo (mentor)
• Mentor: Ana Huđek Turković (pomoć pri izradi, neposredni voditelj)
• Committee member: Marina Krpan (predsjednik povjerenstva)
• Committee member: Ana Butorac (član povjerenstva)
• Committee member: Teuta Murati (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2024-06-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu istraživan je utjecaj pesticida na ekspresiju gena pomoći in silico analize. Pesticidi čiji je utjecaj istraživan su: glifosat, kaptan, spinosad, deltametrin, acetamiprid, nikosulfuron, S-metolaklor i terbutilazin. Upotreba in silico metoda sve je više uznapredovala te se koriste radi relativno brzog prikupljanja podataka o toksičnosti tvari koje nije moguće potpuno ispitati standardnim toksikološkim testovima ili pak za tvari za koje postoje podaci dobiveni standardnim metodama kako bi se provjerila valjanost modela. In silico metode se mogu primijeniti s ciljem relativno brzog dobivanja podataka o toksičnosti supstanci koje nije moguće u potpunosti ispitati standardnim toksikološkim testovima. U radu su korištene dvije online baze podataka: „Comparative toxicogenomics database“ (CTD) i „String“. One sadrže podatke o toksičnom djelovanju pesticida. U ovom radu, usmjerenost je bila na utjacaju pesticida na ekspresiju gena u ljudskom genomu.

Abstract (english)

This study investigates the impact of pesticides on gene expression using in silico analysis. The pesticides investigated include glyphosate, captan, spinosad, deltamethrin, acetamiprid, nicosulfuron, S-metolachlor, and terbuthylazine. The use of in silico methods has advanced significantly, providing quick data collection on the toxicity of substances that cannot be fully examined by standard toxicological tests, or for substances with existing data obtained by standard methods to verify model validity. In silico methods can be applied to obtain relatively fast data on the toxicity of substances that cannot be fully examined by standard toxicological tests. The study utilized two online databases: the Comparative Toxicogenomics Database (CTD) and String, both containing data on the toxic effects of pesticides. The focus of this study was on the impact of pesticides on gene expression in the human genome.

• Keywords: pesticidi; in silico; utjecaj; ekspresija; geni
• Keywords (english): pesticides; in silico; impact; expression; genes
• Language: croatian
• URN:NBN: urn:nbn:hr:159:699723
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-30 13:17:59