Ekstrakcija i karakterizacija bioaktivnih komponenti iz lista planike i ploda maginje (Arbutus unedo L.) s područja otoka Visa

master's thesis

• Title (english): Extraction and characterization of bioactive components from the leaves and fruits of strawberry tree (Arbutus unedo L.) from the island of Vis
• Author: Lana Vujanić
• Mentor: Jasmina Lapić (mentor)
• Mentor: Senka Djaković (pomoć pri izradi, neposredni voditelj)
• Mentor: Anica Bebek Markovinović (pomoć pri izradi, neposredni voditelj)
• Committee member: Senka Djaković (predsjednik povjerenstva)
• Committee member: Danijela Bursać Kovačević (član povjerenstva)
• Committee member: Suzana Rimac Brnčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2024-10-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Obična planika (Arbutus unedo L.), u narodu poznata kao maginja, voćna je vrsta koja posjeduje antioksidacijska i antimikrobna svojstva. Cilj ovog rada bio je izolirati bioaktivne komponente iz lista planike i ploda maginje s područja Visa, pri čemu su korištene sljedeće metode: destilacija vodenom parom, konvencionalna ekstrakcija grijanjem uz povrat, ekstrakcija po Soxhlet-u i ultrazvučna ekstrakcija, uz destiliranu vodu, 70 %-tni etanol i etil-acetat kao ekstrakcijska otapala. Dominantne komponente karakterizirane su pomoću tankoslojne kromatografije (TLC) i IR-spektroskopije. Biljni ekstrakti dobiveni svim ekstrakcijskim metodama korišteni su za spektrofotometrijsko određivanje koncentracije ukupnih fenola, hidroksicimetnih kiselina, flavonola i kondenziranih tanina te za određivanje antioksidacijskog kapaciteta lista i ploda primjenom FRAP i ABTS metoda. Dobiveni rezultati pokazali su da je polifenolni udio značajno veći u ekstraktima lista, u odnosu na ekstrakte ploda te da su najveće masene koncentracije tih spojeva dobivene u 70 %-tnom etanolnom ekstraktu lista dobivenog konvencionalnom ekstrakcijom grijanjem uz povrat.

Abstract (english)

Arbutus unedo L., also known as the strawberry tree, is a fruit species with antioxidant and antimicrobial properties. The aim of this study was to isolate bioactive compounds from the leaves and fruit of the strawberry tree from the island of Vis. The following extraction methods: steam distillation, conventional reflux extraction, Soxhlet extraction, and ultrasonic extraction, with distilled water, 70% ethanol, and ethyl acetate as solvents. The dominant components were characterized using thin-layer chromatography (TLC) and IR spectroscopy. Plant extracts obtained by all extraction methods were used for spectrophotometric determination of total phenols, hydroxycinnamic acids, flavonols, and condensed tannins, as well as for assessing antioxidant capacity using the FRAP and ABTS methods. The results showed that the polyphenolic content was significantly higher in leaf extracts compared to fruit extracts, with the highest concentrations of these compounds found in the 70% ethanol extract obtained through conventional reflux extraction.

• Keywords: maginja; obična planika; bioaktivne komponente; ekstrakcija; polifenoli
• Keywords (english): strawberry tree; Arbutus unedo L. bioactive compounds; extraction; polyphenols
• Language: croatian
• URN:NBN: urn:nbn:hr:159:462120
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-11-25
• Created on: 2024-11-06 14:27:36