Antifungalna aktivnost mikafungina uz primjenu niskotemperaturnih eutektičkih otapala

undergraduate thesis

• Title (english): Antifungal activity of micafungin with the use of therapeutic deep eutectic solvents
• Author: Ema Zubović
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2024-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Mikafungin zbog širokog spektra djelovanja prema različitim vrstama mikroorganizama trenutno je najčešće upotrebljavani antimikotik, a zbog svojeg potencijala i predmet brojnih istraživanja. U sklopu ovog rada ispitana je i uspoređena antifungalna aktivnost čistog mikafungina i mikafungina otopljenog u izabranim eutektičkim otapalima (DES) prilikom čega su dobivena terapeutska niskotemperaturna eutektična otapala s aktivnom tvari (THEDES). Također je određena minimalna inhibitorna koncentracija (MIK) navedenih kombinacija prema izabranim test mikroorganzmima. Dobiveni rezultati su pokazali da su čisti mikafungin kao i THEDES-i uspješno inhibirali plijesni Aspergillus niger i Aspergillus flavus kao i kvasce - Candida albicans, Candida utilis, Candida lypolitica te Candida famata. Otapanjem mikafungina u DES-ovima došlo je do smanjenja minimalne inhibitorne koncentracije kod plijesni A. niger i A. flavus te kod kvasaca C. famata dok kod preostalih ispitanih kvasaca nije zabilježen pozitivan efekt na MIK.

Abstract (english)

Micafungin, due to its broad spectrum of activity against various types of microorganisms, is currently the most commonly used antifungal agent and it is also the subject of numerous studies because of its potential. In this study, the antifungal activity of pure micafungin and micafungin dissolved in selected eutectic solvents (DES) was tested and compared, which resulted and gave therapeutic lowtemperature eutectic solvents with an active ingredient (THEDES). The minimal inhibitory concentration (MIC) of these combinations against selected test microorganisms was also determined. The obtained results showed that both pure micafungin and THEDES successfully inhibited molds Aspergillus niger and Aspergillus flavus, as well as yeasts Candida albicans, Candida utilis, Candida lypolitica, and Candida famata. Dissolved micafungin in DES resulted reduction of the minimal inhibitory concentration for molds A. niger and A. flavus, and for yeast C. famata, while no positive effect on MIC was observed for the remaining tested yeasts.

• Keywords: mikafungin; antifungalna aktivnost; eutektična otapala
• Keywords (english): micafungin; antifungal activity; deep eutectic solvents
• Language: croatian
• URN:NBN: urn:nbn:hr:159:347490
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-08 09:37:25