Bioaktivno djelovanje autohtonih sojeva bakterija mliječne kiseline izoliranih iz kiselog kupusa

undergraduate thesis

• Title (english): Bioactive activity of autochthonous lactic acid bacteria strains isolated from sauerkraut
• Author: Nika Sever
• Mentor: Katarina Butorac (mentor)
• Mentor: Martina Banić (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2024-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterije mliječne kiseline (BMK) su industrijski vrlo važni mikroorganizmi koji se upotrebljavaju kao starter kulture za dobivanje raznih fermentiranih proizvoda. Kao posljedica djelovanja njihovog metabolizma koja ovisi o supstratu i tipu fermentacije mogu sintetizirati metabolite s raznovrsnim bioaktivnim djelovanjem. Cilj ovog rada bio je ispitati potencijalno bioaktivno djelovanje autohtonih sojeva BMK izoliranih iz rasola kiselog kupusa dobivenog spontanom fermentacijom. Potencijal biosinteze makromolekula egzopolisaharida je dokazan kod 33,33 % BMK, dok je najbolje antioksidativno djelovanje dokazano kod dva soja čija je sposobnost uklanjanja DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radikala iznosila oko 37 %. Svi izolirani sojevi su pokazali slabu sposobnost hidrolize kazeina. Antimikrobno djelovanje prema srodnim BMK je bilo slabije izraženo nego prema test-mikroorganizmima. Primijenjeni sojevi imaju potencijal za primjenu u prehrambenoj te biotehnološkoj proizvodnji.

Abstract (english)

Lactic acid bacteria (LAB) are industrially very important microorganisms that are used as starter cultures for the production of various fermented products. As a result of their metabolism, which depends on the substrate and the type of fermentation, they can synthesize metabolites with different bioactive effects. The aim of this work was to investigate the potential bioactive effect of autochthonous LAB strains isolated from sauerkraut obtained by spontaneous fermentation. The potential to biosynthesize exopolysaccharide macromolecules was detected in 33.33 % of LAB, while the best antioxidant activity was detected in two strains whose ability to remove DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical was about 37 %. All isolated strains showed a weak ability to hydrolyze casein. The antimicrobial activity against related LAB was less pronounced than against the test-microorganisms. The strains used have potential for application in food and biotechnological industry.

• Keywords: bakterije mliječne kiseline; bioaktivno djelovanje; kiseli kupus
• Keywords (english): lactic acid bacteria; bioactive activity; sauerkraut
• Language: croatian
• URN:NBN: urn:nbn:hr:159:275016
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-11 14:09:16