Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi

master's thesis

• Title (english): Optimization of cultivating conditions of the yeast Rhodotorula sp. and the thraustochytrid Schizochytrium sp. on seagrass waste
• Author: Nika Morača
• Mentor: Mirela Ivančić Šantek (mentor)
• Committee member: Blaženka Kos (predsjednik povjerenstva)
• Committee member: Tibela Landeka Dragičević (član povjerenstva)
• Committee member: Jasna Novak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2024-11-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sažetak: S obzirom na iscrpljivanje prirodnih resursa poput fosilnih goriva, postoji velika potreba za pronalaskom obnovljivih izvora energije. Lignocelulozna biomasa, kompleksni polimer, obećavajuća je obnovljiva sirovina koja se može koristiti za proizvodnju bioenergetskih i drugih produkata pomoću različitih tipova mikroorganizama. Cilj ovog rada je optimizacija omjera ugljika i dušika (C:N) u hranjivoj podlozi za uzgoj dva oleaginozna mikroorganizma – kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp.. U nastavku istraživanja proveden je uzgoj navedenih mikroorganizama na predobrađenoj otpadnoj travi koja je pretežito sadržavala morsku travu Posidonia oceanica. Najbolji C:N omjer za rast biomase i prinos lipida bio je 100 g/g za kvasac te 3,75 g/g za traustohitrid. Uzgojem kvasca na predobrađenoj morskoj travi proizvedeno je 73,4 g lipida/kg sirovine, a uzgojem traustohitrida 132,2 g lipida/kg sirovine.

Abstract (english)

Abstract: As natural resources such as fossil fuels deplete, there is an urgent need to find sustainable energy sources. Lignocellulose, a complex polymer, has emerged as a promising renewable resource which can be used for production of bioenergetic and other products using different kinds of microorganisms. The goal of this thesis is optimization of carbon to nitrogen (C:N) ratio for cultivation of two oleaginous microorganisms – yeast Rhodotorula sp. and thraustochytrid Schizochytrium sp. using the marine seagrass Posidonia oceanica as a substrate. In the following research, the cultivation of mentioned microorganisms was carried out on pretreated waste grass, which predominantly contained Posidonia oceanica. The best C:N ratio for biomass growth and lipid yield was 100 g/g for the yeast and 3,75 g/g for the thraustochytrid. Cultivation of yeast on pretreated sea grass yielded 73,4 g of lipids/kg of raw material and cultivation of thraustochytrid yielded 132,2 g of lipids/kg of raw material.

• Keywords: omjer C/N; Rhodotorula; Schizochytrium; lipidi; morska trava
• Keywords (english): C/N ratio; Rhodotorula; Schizochytrium; lipids; sea grass
• Language: croatian
• URN:NBN: urn:nbn:hr:159:204218
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-04 09:58:58