Bioaktivni potencijal i sastav ugljikohidratne frakcije obične trske (Phragmites australis)

master's thesis

• Title (english): Bioactive potential and carbohydrate fraction composition of common reed (Phragmites australis)
• Author: Karla Zrnić
• Mentor: Aleksandra Vojvodić Cebin (mentor)
• Committee member: Božidar Šantek (predsjednik povjerenstva)
• Committee member: Draženka Komes (član povjerenstva)
• Committee member: Antonija Trontel (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Chemistry and Technology of Carbohydrates and Confectionery Products); Zagreb
• Defense date and country: 2024-12-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Obična trska (Phragmites australis) je biljka vodenih staništa koja je kroz povijest korištena u razne svrhe. Danas se sve više istražuje njezin potencijal u proizvodnji bioplina, za pročišćavanje otpadnih voda i bioremedijaciju tla. Cilj ovog rada bio je karakterizirati polifenolnu i ugljikohidratnu frakciju obične trske. Provedena je ekstrakcija različitih dijelova biljke (cijela trska, cvijet, list i stabljika) te su određeni udjeli ukupnih i pojedinačnih polifenola (slobodni i vezani) te antioksidacijski kapacitet. Analizom monomernog profila te udjela vlakana, dobiven je uvid u sastav i udio strukturnih ugljikohidrata. Polifenolni profil svih dijelova trske bio je jednak te su određeni rutin, kvercetin-3-glukozid i kvercetin, čiji udio je bio najveći u listu trske, a najmanji u stabljici trske. Dodatak etanola pozitivno je utjecao na ekstrakcijski prinos navedenih spojeva. Nestrukturne ugljikohidrate činili su glukoza, fruktoza i maltoza, u ukupnom udjelu od 4,6 % s.tv. Hemiceluloza arabinoksilanskog tipa je bila najzastupljeniji strukturni polisaharid (~26 % s.tv.).

Abstract (english)

Common reed (Phragmites australis) is a wetland plant and has been used for various purposes throughout history. Nowadays, its potential in the production of biofuels, wastewater purification, and soil bioremediation has been increasing. This work aimed to characterize the polyphenolic and carbohydrate fractions of common reed. Different parts of the plant (whole reed, flower, leaf and stem), were analyzed for total and individual phenolics (free and bound) and antioxidant capacity, upon extraction. Monomeric composition profiling and fibre analysis enabled the determination of nonstructural and structural carbohydrates. The analyzed parts of the reed did not differ with respect to the polyphenolic profile. Rutin, quercetin-3-glucoside and quercetin were identified, exhibiting the highest content in reed leaf while the lowest in reed stem. The addition of ethanol enhanced the extractability of these compounds. Nonstructural carbohydrates were comprised of glucose, fructose and maltose, reaching 4,6 % dmb. Arabinoxylan-type hemicellulose was the most abundant hemicellulosic polysaccharide (~26 % dry matter).

• Keywords: ekstrakcija; obična trska; Phragmites australis; polifenolni spojevi; strukturni ugljikohidrati
• Keywords (english): extraction; common reed; Phragmites australis; polyphenolic compounds; structural carbohydrates
• Language: croatian
• URN:NBN: urn:nbn:hr:159:979547
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-18 10:47:51