Određivanje citotoksičnog, prooksidacijskog i genotoksičnog učinka acetamiprida, nikosulfurona, S-metolaklora i terbutilazina na humanim kontinuiranim staničnim linijama želuca i jetre

master's thesis

• Title (english): Determination of cytotoxic, prooxidative and the genotoxic effect of acetamiprid, nicosulfuron, S-metolachlor and terbuthylazine on human continuous gastric and liver cell lines
• Author: Mariana Lenček
• Mentor: Ksenija Durgo (mentor)
• Mentor: Ana Huđek Turković (pomoć pri izradi, neposredni voditelj)
• Committee member: Marina Krpan (predsjednik povjerenstva)
• Committee member: Nevenka Kopjar (član povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2024-12-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Pesticidi značajno doprinose poljoprivrednoj produktivnosti, no prekomjerna uporaba dovela je do njihove akumulacije u okolišu. Nakupljaju se u podzemnim vodama, zraku, zemlji pa tako i u hrani. Na taj način dolaze u kontakt s mnogim neciljanim organizmima među kojima su i ljudi. Zbog toga je od iznimne važnosti pratiti potencijalne toksične učinke pesticida te njihovih metabolita na ljudsko zdravlje. Dugotrajna izloženost pesticidima povezana je s raznim zdravstvenim problemima, uključujući endokrine poremećaje, neurotoksičnost i karcinome. U ovom istraživanju ispitani su citotoksični, prooksidacijski i genotoksični učinci četiri pesticida (acetamiprid, nikosulfuron, S-metolaklor i terbutilazin) na humanim staničnim linijama hepatocelularnog karcinoma jetre (Hep G2) i adenokarcinoma epitela želuca (AGS). Ispitivane su koncentracije koje mogu biti prisutne u okolišu, omogućujući analizu realnog rizika za ljudsko zdravlje. Acetamiprid, S-metolaklor i nikosulfuron djelovali su proliferativno na Hep G2, a acetamiprid i terbutilazin na AGS staničnu liniju. Terbutilazin pri niskim koncentracijama djeluje prooksidacijski na AGS staničnu liniju te pokazuje prooksidacijsko djelovanje u prisutnosti hidroksilnih radikala pri većim koncentracijama. Na staničnoj kulturi AGS acetamiprid, terbutilazin i S-metolaklor pokazuju genotoksičan potencijal.

Abstract (english)

Although pesticides significantly contribute to agricultural productivity, their excessive use has led to their accumulation in the environment. They can be found in groundwater, air, soil and therefore in food. This way they can come in contact with many non-target organisms, including humans. For this reason, it is crucial to monitor the potential toxic effects of pesticides on human health. Long-term exposure to pesticides has been associated with various health problems, including endocrine disorders, neurotoxicity, and cancer. In this study, the cytotoxic, pro-oxidative, and genotoxic effects of four pesticides (acetamiprid, nicosulfuron, S-metolachlor, and terbuthylazine) were examined on human cell lines of liver hepatocellular carcinoma (Hep G2) and stomach epithelial adenocarcinoma (AGS). Tested concentrations correspond to possible concentrations found in environment, allowing us to estimate realistic risk to human health. The results provide insight into the potential toxic effects of pesticides on the human digestive and metabolic systems. Acetamiprid, S-metolachlor and nicosulfuron had a proliferative effect on Hep G2, and acetamiprid and terbuthylazine on the AGS cell line. At low concentrations, terbuthylazine has a pro-oxidative effect on the AGS cell line and shows a pro-oxidative effect in the presence of hydroxyl radicals at higher concentrations. On AGS cell culture, acetamiprid, terbuthylazine and S-metolachlor show genotoxic potential.

• Keywords: pesticidi; AGS i Hep G2 stanične linije; genotoksičnost; citotoksičnost; crijevni mikrobiom
• Keywords (english): pesticides; AGS and Hep G2 cell lines; genotoxicity; cytotoxicity; gut microbiota
• Language: croatian
• URN:NBN: urn:nbn:hr:159:385282
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-13 13:59:10