Biološka aktivnost derivata ciprofloksacina određena na HeLa i HaCaT staničnim linijama

master's thesis

• Title (english): Biological activity of ciprofloxacin derivatives determined on HeLa and HaCaT cell lines
• Author: Nikolina Teskera
• Mentor: Kristina Radošević (mentor)
• Mentor: Martina Bagović Kolić (pomoć pri izradi, neposredni voditelj)
• Committee member: Ksenija Markov (predsjednik povjerenstva)
• Committee member: Senka Djaković (član povjerenstva)
• Committee member: Jasmina Lapić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); Zagreb
• Defense date and country: 2024-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ciprofloksacin osim antibakterijske aktivnosti posjeduje i antitumorski potencijal. Iz tog razloga je u ovom radu ispitana in vitro aktivnost triju derivata ciprofloksacina koji u svojoj strukturi sadrže molekulu ferocena čijom konjugacijom nastaju spojevi koje odlikuje povećana bioaktivnost. Antitumorski učinak ispitan je na HeLa i HaCaT stanicama praćenjem stanične vijabilnosti i klonogenom analizom, dok je antimikrobna aktivnost određena disk-difuzijskom metodom. Ispitani derivati imaju inhibitorni učinak na rast HeLa i HaCaT stanica, ali su djelotvorniji na HeLa stanicama. Pri tome najjače citotoksično djelovanje pokazuje spoj XIV-39, a najslabije XIV-51, što je potvrđeno klonogenom analizom. Svi derivati posjeduju antibakterijsku aktivnost, a najučinkovitijim se istaknuo XIV-51. Iz navedenog se može zaključiti da su derivati ciprofloksacina zadržali antimikrobnu aktivnost te da imaju antitumorski potencijal.

Abstract (english)

Ciprofloxacin, in addition to its antibacterial activity, also exhibits antitumor potential. For this reason, this study investigated the in vitro activity of three ciprofloxacin derivatives that incorporate a ferrocene molecule into their structure, resulting in conjugates characterized by enhanced bioactivity. The antitumor effect was evaluated on HeLa and HaCaT cells by assessing cell viability and performing clonogenic assays, while the antimicrobial activity was determined using the disk diffusion method. The tested derivatives demonstrated inhibitory effects on the growth of HeLa and HaCaT cells, with greater efficacy observed on HeLa cells. Among them, compound XIV-39 exhibited the strongest cytotoxic effect, while XIV-51 showed the weakest, as confirmed by clonogenic analysis. All derivatives retained antibacterial activity, with XIV-51 being the most effective. Therefore, it can be concluded that ciprofloxacin derivatives maintain their antimicrobial properties and have potential as antitumor agents.

• Keywords: derivati ciprofloksacina; in vitro testovi; citotoksičnost; HeLa stanična linija
• Keywords (english): ciprofloxacin derivatives; in vitro tests; cytotoxicity; HeLa cell line
• Language: croatian
• URN:NBN: urn:nbn:hr:159:787848
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-13 14:16:00