Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis

master's thesis

• Title (english): The effect of hydrogen peroxide on the heterotrophic cultivation of alfae Euglena gracilis
• Author: Ena Jurković
• Mentor: Božidar Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2014-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom istraživanju proučavan je heterotrofni uzgoj alge Euglena gracilis s ciljem definiranja optimalnog sastava hranjive podloge za industrijsku proizvodnju biomase i paramylona. Tijekom uzgoja na laboratorijskoj tresilici i u bioreaktoru s mješalom ispitivan je heterotrofni uzgoj alge E. gracilis na kemijski definiranoj (modificirana Hutnerova podloga) i kompleksnoj podlozi sa i bez dodatka vodikovog peroksida, te sa i bez kontaminacije kvascem Saccharomyces cerevisiae i bakterijom Bacillus subtilis. Kompleksna hranjiva podloga (20 g/L glukoze + 60 g/L CSL) primijenjena je s ciljem smanjenja troškova proizvodnje biomase i paramylona u velikom mjerilu. Na osnovi rezultata istraživanja vidljivo je da kompleksna hranjiva podloga može uspješno zamijeniti kemijski definiranu Hutner-ovu podlogu. Stoga kompleksna hranjiva podloga ima veliki potencijal za primjenu u industrijskoj proizvodnji. Nadalje, kod heterotrofnog uzgoja na obje hranjive podloge uočeno je da 5 mg/L H2O2 ima značajan utjecaj na dinamiku odvijanja bioprocesa. Navedena koncentracija H2O2 uspješno sprječava rast kontaminanata (S. cerevisiae i B. subtilis), a što je dokazano uzgojem u Erlenmeyer tikvicama i u bioreaktoru s mješalom.

Abstract (english)

In this research, the heterotrophic cultivation of algae Euglena gracilis was studied in order to define the optimum composition of culture media for the industrial production of biomass and paramylon. During the heterotrophic cultivation on the laboratory shaker and stirred tank bioreactor, the chemically defined (modified Hutner medium) and a complex medium with and without the addition of hydrogen peroxide, and with and without contamination of the yeast Saccharomyces cerevisiae and the bacterium Bacillus subtilis, were examined. The complex nutrient medium (20 g/L glucose + 60 g/L CSL) was applied in order to reduce production costs on a large scale. Based on the results of research, it is evident that a complex nutrient medium can successfully replace chemically defined Hutner medium. Therefore, a complex nutrient medium has a great potential for application in industrial production. Furthermore, in heterotrophic cultivation on both culture media was observed that 5 mg/L H2O2 has a significant impact on the dynamics of bioprocess. The above mentioned concentration of H2O2 successfully prevents the growth of contaminants (S. cerevisiae and B. subtilis), which is proven by cultivation in Erlenmeyer flasks and stirred tank bioreactor.

• Keywords: Euglena gracilis; heterotrofni uzgoj; vodikov peroksid; kompleksna hranjiva podloga; β-1; 3-glukan (paramylon)
• Keywords (english): Euglena gracilis; heterotrophic cultivation; hydrogen peroxide; complex growth medium; β-1; 3-glucan (paramylon)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:055141
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 12:25:32