Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A

master's thesis

• Title (english): Noble mould Penicillium nalgiovense as possible producent of aflatoxin B1 and ochratoxin A
• Author: Jelena Pugelnik
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2014-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Plemenita plijesan Penicillium nalgiovense ima biotehnološku važnost u prehrambenoj industriji (suhomesnati proizvodi) zbog svoje široke upotrebe kao starter kultura. Obzirom da plijesni sintetiziraju sekundarne toksične metabolite mikotoksine, suhomesnati proizvodi predstavljaju potencijalnu opasnost po zdravlje potrošača. Svrha ovog rada bio je istražiti može li liofilizirana kultura plemenite plijesni Penicillium nalgiovense i soj plijesni Penicillium sp. 622 koje se koriste u industrijskoj proizvodnji zimske salame, pri odabranim uvjetima uzgoja, sintetizirati aflatoksin B1 (AFB1) i okratoksin A (OTA). Za istraživanje prisutnosti mikotoksina u uzorcima upotrijebljena je tankoslojna kromatografija, a koncentracija AFB1 i OTA je određena ELISA (Enzyme Linked Immuno Sorbent Assay) metodom. Rezultati istraživanja su pokazali da je AFB1 dokazan u 72,7 %, a OTA u 100% istraživanih uzoraka. Koncentracije AFB1 bile su u rasponu od nd (nije dokazano) do 0,125 pg×mL-1, dok je najveća količina OTA od 4,0 pg×mL-1 dokazana u liofilizatu komercijalne starter kulture P. nalgiovense.

Abstract (english)

The noble mold Penicillium nalgiovense has a biotechnological importance in the food industry ( meat products ), because of its wide use as starter culture. Since mold synthesizes toxic secondary metabolites, mycotoxins, meat products poses a potential threat to abusers health. The purpose of this paper was to investigate whether the lyophilized cultures of noble Penicillium nalgiovense and strain of Penicillium sp. 622, which are used in the industrial production of winter salami, in chosen growing conditions, synthesizes aflatoxin B1 (AFB1) and ochratoxin A (OTA). The presence of mycotoxins in the samples was determined by thin layer chromatography, and the concentration of AFB1 and OTA was determined by qualitative ELISA ( Enzyme Linked Immuno Sorbent Assay ) method. The study results showed that AFB1 was detected in 72.7% and OTA in 100% of the samples. AFB1 concentrations ranged from ND (not detected ) to 0,125 pg × ml-1, while the maximum amount of OTA (4.0 pg × ml-1) was detected in the lyophilized commercial starter culture of P. nalgiovense.

• Keywords: plemenite plijesni; Penicillium nalgiovense; aflatoksin B1; okratoksin A
• Keywords (english): noble mold; Penicillium nalgiovense; aflatoxin B1; ochratoxin A
• Language: croatian
• URN:NBN: urn:nbn:hr:159:540934
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 12:54:15