Ekstrakcija fenolnih spojeva iz pokožice grožđa primjenom eutektičnih otapala

master's thesis

• Title (english): Application of deep eutectic solvents in extraction of phenolic compounds from grape skin
• Author: Ivana Šolčić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2014-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Pokožica grožđa smatra se otpadom koji nastaje u procesu proizvodnje vina, pa tako i pokožica Plavca malog, koja je bogata fenolnim spojevima, koji su se pokazali kao snažni antioksidansi, te se kao takvi mogu koristiti kao dodaci prehrani. Iz tog je razloga potrebno primijeniti proces „zelene“ ekstrakcije, te su se u tom području kao dobar izbor pokazala eutektična otapala koja su ekološki u potpunosti prihvatljiva i ne djeluju štetno na ljudsko zdravlje. Kao najučinkovitije otapalo za ekstrakciju ukupnih fenolnih spojeva, flavan-3-ola, flavonola i antocijana pokazalo se otapalo sastavljeno od kolin klorida i oksalne kiseline sa udjelom vode od 25 %. U ekstrakciji fenolnih kiselina najbolje je bilo otapalo sastavljeno od kolin klorida i sorboze s udjelom vode od 50 %. Najzastupljeniji antocijan je malvidin-3-glukozid, kojeg je najviše ekstrahirano sa eutektičnim otapalom sastavljenim od kolin klorida i oksalne kiseline sa 25 % vode u otapalu. Eutektična otapala pokazala su se učinkovitija u ekstrakciji fenolnih spojeva u odnosu na klasičnu ekstrakciju metanolom i zakiseljenim metanolom, što nam ukazuje na mogućnost potencijalne zamjene klasičnih organskih otapala eutektičnim otapalima u ekstrakciji fenolnih spojeva iz pokožice grožđa i drugih biljnih materijala.

Abstract (english)

Grape skin is considered waste generated in the wine-making process, including the skin of grape Plavac mali, rich in phenolic compounds, which have proved to be powerful antioxidants, and as such can be used as nutritional supplements or pharmaceuticals. For this reason it is necessary to apply the process of "green" extraction, in which area eutectic solvents are good option,completely ecologically acceptable and do not adversely affect human health. As the most effective solvent for the extraction of total phenolic compounds, flavan-3-ols, flavonols and anthocyanins was a solvent composed of choline chloride and oxalic acid with a water content of 25%. The extraction of the phenolic acids was the best with solvent composed of choline chloride and sorbose with a water content of 50%. The most common anthocyanin is malvidin-3-glucoside, which is the best extracted with eutectic solvent composed of choline chloride and oxalic acid with 25% of water in the solvent. Eutectic solvents have proved to be effective in the extraction of phenolic compounds compared to conventional extraction with methanol and acidified methanol, which indicates the possibility of a potential replacement of conventional organic solvents with eutectic solvents in the extraction of phenolic compounds from the grape skin and other plant material.

• Keywords: antocijani; ekstrakcija; eutektična otapala; fenolni spojevi; pokožica grožđa
• Keywords (english): anthocyanins; extraction; deep eutectic solvents; phenols; grape skin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:941608
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 10:39:06