Priprava ekstrakta potkožice grožđa primjenom eutektičnih otapala i ispitivanje njihove biološke aktivnosti

master's thesis

• Title (english): Preparation of grape berry skin extract using eutectic solvents and testing their biological activity
• Author: Ana-Marija Pogačić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2014-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Brojne epidemiološke studije ukazuju na povezanost konzumiranja voćnih plodova i smanjenja rizika od različitih tumorskih i drugih oboljenja u ljudi. Eutektična otapala su otopine sintetizirane iz dva ili više spoja koji se međusobno mogu povezati vodikovim vezama,a jedno od područja njihove primjene je ekstrakcija bioaktivnih spojeva iz biljnog materijala. Stoga su u ovom radu pripremljeni ekstrakti pokožice grožđa sa pet različitih eutektičnih otapala i metanolom te je analiziran njihov sastav obzirom na pojedinačne i ukupne antocijane, fenole i flavonoide te je određen njihov antioksidacijski kapacitet. Biološka aktivnost pripremljenih ekstrakta pokožice grožđa ispitana je in vitro na trima staničnim linijama (MCF-7, HeLa, CHO-K1). Najizraženiji inhibitorni učinak na tumorske stanične linije MCF-7 i HeLa imao je ekstrakt pokožice grožđa u kolin-klorid:ksiloza koji je imao visoke vrijednosti masenih udjela fenola, flavonoida, antocijana te antioksidativng kapaciteta. Ekstrakt u kolin-klorid:glicerol i metanolni ekstrakt nemaju citotoksični učinak na rast tumorskih staničnih linija, a navedeni ekstrakti imaju najniže vrijednosti masenih udjela fenola, flavonoida i antocijana te antioksidativne kapacitete. Na CHO-K1 staničnu liniju nijedan od ispitivanih ekstrakata nije imao citotoksičan učinak.

Abstract (english)

Numerous epidemiological studies indicate association between the consumption of fruit berries with a reduced risk from different tumor and other diseases in human. Deep eutectic solvents are fluids generally composed of two or tree cheap and safe components that are capable of selfassociation through hydrogen bond interactions, and one of the fields of application is extraction of bioactive compounds from plants. Therefore, in this paper, grape epidermis extracts are prepared from five different deep eutectic solvents and methanol and their composition is analyzed considering the individual and total anthocyanins, phenols and flavonoids and their antioxidant capacity is determined. The biological activity of the prepared extract of grape berry skin is examined in vitro on three cell lines (MCF-7, HeLa, CHO-K1). The highest inhibitory effect on tumor cell lines MCF-7 and HeLa had the extract in choline.chloride-xylose which had high shares of phenols, flavonoids, anthocyanins and antioxidant capacity. Choline-chloride: glycerol extract and methanol extract did not have cytotoxic effect on the growth of tumor cell lines, and these extracts have the lowest shares of phenols, flavonoids, anthocyanins and antioxidant capacity. On CHO-K1 cell line none of these extracts did not have cytotoxic effect.

• Keywords: antioksidacijski kapacitet; antocijani; biološka aktivnost; eutektična otapala; fenoli; flavonoidi
• Keywords (english): antioxidative capacity; anthocyanins; biological activity; deep eutectic solvents; flavonoids; phenols
• Language: croatian
• URN:NBN: urn:nbn:hr:159:271918
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 12:47:06