Antioksidacijski učinak bioaktivnih spojeva iz ekstrakta pokožice grožđa na hiperkolesterolemiju u miša

master's thesis

• Title (english): Antioxidant effects of bioactive compounds from the extracts of the berry skin of grapes on hypercholesterolemia in mice
• Author: Sandra Zugan
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2015-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Ekstrakt pokožice grožđa obiluje polifenolima - skupinom organskih spojeva koji imaju antioksidacijsko djelovanje, odnosno smanjuju oksidacijski stres. Reciklaža predstavlja odlično ekološko rješenje tretiranja vinskog organskog otpada budući da se godišnje izdvaja tisuću tona pokožice tijekom vinifikacije. U ovom radu istražen je zaštini učinak bioaktivnih spojeva iz ekstrakta pokožice grožđa na oksidacijski stres potaknut hiperlipidnom prehranom u miša. Istraživani biomarkeri oksidacijskog stresa u jetri i bubrezima bili su malonildialdehid (MDA), karbonil proteini (PC), superoksid dismutaza (SOD), katalaza (CAT) i reducirani glutation (GSH). Rezultati pokazuju da je tretman s ekstraktom pokožice grožđa (GSE) u dozi od 100 mg kg-1 tjelesne mase statistički značajno (ANOVA, p<0,05) smanjio aktivnosti PC i MDA u jetri i bubrezima mužjaka i ženki te povećao koncentracije SOD, CAT, GSH u odnosu na kontrolnu skupinu životinja što se može pripisati bioaktivnim spojevima prisutnim u GSE. Dodavanjem ekstrakta pokožice grožđa prehrambenim proizvodima mogla bi se proizvoditi funkcionalna hrana s kardioprotektivnim učinkom.

Abstract (english)

The grape skin extract is rich in polyphenols - a group of organic compounds that have antioxidant activity and thus reduce oxidative stress. Recycling represents an excellent ecological solution for treating vine organic waste since every year over a thousand tons of grape skin is segregated during vinification. In this paper, we studied the effect of protective bioactive compounds from the grape skin extract on oxidative stress induced by high fat diet in mice. Investigated biomarkers of oxidative stress in the liver and kidneys were: malondialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH). The results show that the treatment with the grape skin extract (GSE) in a dose of 100 mg kg-1 body weight significantly (ANOVA, p<0,05) reduced the activity of the PC and MDA in the liver and kidneys of both male and female mice, and increased concentrations of SOD, CAT and GSH compared to the control group which can be attributed to the bioactive compounds present in the GSE. By adding grape skin extract to food we could produce functional food with cardioprotective effect.

• Keywords: pokožica grožđa; oksidacijski stres; lipidna peroksidacija; superoksid dismutaza; reducirani glutation
• Keywords (english): grape skin; oxidative stress; lipid peroxidation; superoxide dismutase; reduced glutathione
• Language: croatian
• URN:NBN: urn:nbn:hr:159:092057
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 08:05:18