Učinak resveratrola na vijabilnost CHO (Chinese Hamster Ovary) - K1 stanica uz ili bez tretmana polikloriranim bifenilima

master's thesis

• Title (english): Effect of resveratrol on CHO (Chinese Hamster Ovary) - K1 cells with or without polychlorinated biphenyls treatment
• Author: Sanja Marđetko
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2015-02-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitan je učinak različitih koncentracija (2,5 - 150 M) resveratrola na CHO (Chinese Hamster Ovary)-K1 staničnoj liniji. Neutral Red metodom određeno je statistički značajno povećanje stanične vijabilnosti pri koncentracijama 5, 10, 20, 35, 50 μM, dok je pri višim koncentracijama resveratrola (100 i 150 μM) utvrđeno statistički značajno smanjenje vijabilnosti stanica. Trypan Blue metodom utvrđeno je statistički značajno smanjenje stanične proliferacije pri svim primijenjenim koncentracijama resveratrola. Ispitivanje mogućeg protektivnog djelovanja antioksidansa resveratrola na vijabilnost stanica tretiranih sa 75 μM PCB-153 pokazalo je njegov blagi protektivni učinak pri koncentracijama od 10, 20 i 35 μM, međutim taj učinak nije bio statistički značajan, dok takvo protektivno djelovanje antioksidansa nije primijećeno pri tretmanu stanica s planarnim PCB-77. Prilikom tretmana CHO-K1 stanica resveratrolom utvrđeno je da sam resveratrol inducira formiranje reaktivnih kisikovih vrsta, dok u kombinaciji s PCB kongenerima ne smanjuje njihovu produkciju, odnosno ne djeluje kao tipični antioksidans.

Abstract (english)

In this study the effects of different concentrations (2,5 - 150 M) of resveratrol on CHO (Chinese Hamster Ovary)-K1 cell line were determined. Statistically significant increase in cell viability was detected by Neutral Red method, when lower concentrations of resveratrol (5, 10, 20, 35 and 50 μM) were applied. Higher concentrations of resveratrol (100 and 150 μM) caused statistically significant decrease in cell viability. Decrease in cell proliferation was found at all applied concentrations of resveratrol by using Trypan Blue method. The study of possible protective impact of antioxidant resveratrol on the viability of cells treated with 75 μM PCB-153 showed that resveratrol has a small protective effect at concentrations 10, 20 and 35 μM, but the extent of effect was not statistically significant, while such protective effect is not observed when cells were teated with planar PCB-77. Resveratrol induces formation of reactive oxygen species, while in combination with PCB congeners does not affect their production, thus does not exert typical antioxidative activity.

• Keywords: PCB-77; PCB-153; CHO-K1 stanična linija; resveratrol; citotoksičnost
• Keywords (english): PCB-77; PCB-153; CHO-K1 cell line; resveratrol; cytotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:012062
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 08:32:19