Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije

master's thesis

• Title (english): Analysis of process kinetic of PHB biosynthesis in continuous production using the cascade of 5 biorectors and electron microscopy
• Author: Denis Vadlja
• Mentor: Predrag Horvat (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2015-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom istraživanju analiziran je peterostupanjski reaktorski sustav za kontinuiranu proizvodnju PHB-a. Korištena je skenirajuća transmisijska elektronska mikroskopija (STEM). Programski paket ImageJ primijenjen je za analizu veličine stanica i unutarstaničnih poli-(3-hidroksibutirat) (PHB) granula. Primijenjena je tehnika digitalne analize STEM snimaka uz mjerenje tlocrtne površine stanica i PHB granula (pod pretpostavkom da između površine na slici i realnog volumena objekta postoji direktna proporcionalnost). Specifične brzine rasta (μ) i specifične brzine nastajanja PHB-a (qP) za kulturu Cupriavidus necator DSM 545 određene su gravimetrijski i pomoću odnosa tlocrtnih površina stanica i PHB granula za svih pet reaktora. Dobiveni rezultati uspoređeni su međusobno. Na temelju dobivenih rezultata, zaključeno je da se ImageJ program može uspješno koristiti za određivanje veličine stanica i granula mjerenjem tlocrtne površine istih na STEM snimkama. Specifična brzina rasta (μ) određena mjerenjem tlocrtne površine stanica slaže se sa specifičnom brzinom rasta (μ) određenom gravimetrijski.

Abstract (english)

In this study, a 5-step reactor system for the production of PHB was analyzed. Scanning transmission electron microscopy (STEM) and ImageJ software package were used to anaylize the cell size and the size of intracellular poly-3-hydroxybutyrate (PHB) granules. Digital imaging technique (analysis) of the STEM images was applied for the measuring of cells and PHB-granula areas (assuming that there is a direct proportionality between the image area and the related real object volume). The specific growth rate (μ) and the specific PHB productivity rate (qp) for Cupriavidus necator DSM 545 culture were determined by gravimetric and by digital imaging technique analysis for all five reactors in cascade. The results were compared mutually. Based on the obtained results, it is evident that the ImageJ software package can be successfully used for the determination of cells and PHB-granula areas present in the STEM images. The specific growth rate (μ) determined by digital imaging technique analysis matches very well with those determined by gravimetric method.

• Keywords: Cupriavidus necator; ImageJ; peterostupanjski reaktorski sustav; PHB; STEM
• Keywords (english): Cupriavidus necator; five-step reactor system; ImageJ; PHB; STEM
• Language: croatian
• URN:NBN: urn:nbn:hr:159:707664
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 09:31:17