Određivanje glukozinolata u sjemenu uljane repice

undergraduate thesis

• Title (english): Determination of glucosinolates in rapeseed
• Author: Doris Delač
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2015-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Uljana repica je biljka iz porodice Brassicaceae te se koristi za proizvodnju ulja i biodisela. Nusproizvod koji nastaje nakon ekstrakcije ulja iz sjemena uljane repice je sačma koja se najčešće koristi kao hrana za životinje. Kako bi se sačma mogla koristiti kao hrana za životinje, mora sadržavati niske koncentracije glukozinolata. Glukozinolati su spojevi tioglikozidne strukture koji svojom razgradnjom daju spojeve s različitim biološkim djelovanjem. Glukozinolati imaju mnoge pozitivne učinke, međutim posebno je važno napomenuti da prevelika koncentracija razgradnih produkata glukozinolata u sačmi koja se koristi za ishranu životinje uzrokuje gušavost. Analizirano 13 različitih uzoraka sjemena uljane repice s ciljem identifikacije i kvantifikacije glukozinolata. Analizu smo proveli u skladu sa standardnom metodom za identifikaciju glukozinolata koja uključuje ekstrakciju s metanolom, desulfataciju te određivanje udjela glukozinolata tekućinskom kromatografijom visoke djelotvornosti obrnutih faza. Udio ukupnih glukozinolata je u svih 13 uzoraka manji od 20 mol g-1 što je unutar dozvoljenih granica da bi se sačma mogla koristiti za prehranu životinja. Progoitrin je najzastupljeniji u svim ispitivanim uzorcima.

Abstract (english)

Oilseed rape is a plant from Brassicaceae family used for oil and biodiesel production. By-product of oil extraction is rapeseed meal usually used for animal feed. For rapeseed meal to be used as animal food it must contain low amounts of glucosinolate. Glucosinolates are compounds of tioglycoside structure which, by degradation, form compounds with various biological activities. Glucosinolates have many positive effects but it is important to point out that too high concentration of glucosinolate rapeseed meal used as animal food can cause goitre. We analyzed glucosinolates in 13 different rapeseed samples. by HPLC standard glucosinolate method which includes extraction with methanol, desulphatation and content determination. Concentrations of total glucosinolates were lower than 20 μmol g-1 in all samples which was below allowed content for rapeseed meal and ti could be used as animal food. Progoitrin was the most abound one in all samples.

• Keywords: uljana repica; glukozinolati; sačma; tekućinska kromatografija visoke djelotvornosti
• Keywords (english): rapeseed; glucosinolates; meal; liquid chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:159:836158
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-05 12:00:55