Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi

master's thesis

• Title (english): Genotoxic effect of β-adrenergic agonist salbutamol on human lymphocytes, liver cells and blood of guinea pigs
• Author: Maja Ereš
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2012-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Salbutamol je β2-adrenergički agonist koji se u terapeutskim dozama koristi za liječenje kronične opstruktivne bolesti pluća, posebno astme. U dozama 5-10 puta većim od terapeutskih djeluje kao promotor rasta stoke te predstavlja potencijalnu opasnost za zdravlje ljudi i životinja. Cilj ovog rada bio je utvrditi potencijal salbutamola da izazove lomove DNA u tkivu jetre i limfocitima zamorčadi tretiranih salbutamolom (0,25 mg kg-1 i 2,5 mg kg-1 tjelesne mase) te utvrditi njegov genotoksični učinak u rasponu koncentracija od 0,8 µg L-1 do 2,5 mg L-1 na ljudskim limfocitima u in vitro uvjetima uz dodatak metaboličkog aktivatora. Salbutamol u in vivo uvjetima pokazuje antioksidacijsko djelovanje, prvenstveno u stanicama limfocita tretiranih životinja. In vitro salbutamol u koncentraciji od 0,8 µg L-1 djeluje genotoksično.

Abstract (english)

Salbutamol is β2-adrenergic agonist which is used in therapeutic doses for the treatment of chronic obstructive pulmonary diseases, especially asthma. In doses 5-10 times higher than the therapeutic one, it acts as a growth promotor in livestock and as such represents a potential health hazard to humans and animals. In this paper, genotoxic properties of salbutamol were identified; its ability to cause DNA breakage in liver tissue and lymphocytes of guinea pigs (0,25 and 2,5 mg kg-1 of body weight) and DNA damage in human lymphocytes (0,8 to 2,5 mg L-1) with addition of metabolic activator. Salbutamol in vivo reduces DNA damage in the liver and blood cells compared to control, with the apparent reduction in the lymphocytes of treated animals. In vitro, salbutamol at a concentration of 0,8 mg L-1 showed extreme genotoxic effect in comparison to the control.

• Keywords: salbutamol; genotoksičnost ß2-adrenergičkih agonista; komet test
• Keywords (english): salbutamol; ß2-adrenergic agonists genotoxicity; commet assay
• Language: croatian
• URN:NBN: urn:nbn:hr:159:140716
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-08 14:00:03